Back to Search
Start Over
Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
- Source :
- The Immune Synapse ISBN: 9781071631348, Methods in Molecular Biology, Methods in Molecular Biology-The Immune Synapse
- Publication Year :
- 2023
- Publisher :
- Springer US, 2023.
-
Abstract
- The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subsequent monitoring of their traffic toward acceptor compartments. Here we describe the RUSH system applied to cytotoxic T cells to characterize the biogenesis of lytic granules, using as a proof-of-concept granzyme B trafficking to this specialized compartment.
Details
- ISBN :
- 978-1-07-163134-8
978-1-07-163135-5 - ISSN :
- 10643745 and 19406029
- ISBNs :
- 9781071631348 and 9781071631355
- Database :
- OpenAIRE
- Journal :
- The Immune Synapse ISBN: 9781071631348, Methods in Molecular Biology, Methods in Molecular Biology-The Immune Synapse
- Accession number :
- edsair.doi.dedup.....291a8706241fb7fdc6ed4a2cd68e60a1
- Full Text :
- https://doi.org/10.1007/978-1-0716-3135-5_27