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Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes

Authors :
Capitani, Nagaja
Cassioli, Chiara
Ravichandran, Keerthana
Baldari, Cosima T.
Source :
The Immune Synapse ISBN: 9781071631348, Methods in Molecular Biology, Methods in Molecular Biology-The Immune Synapse
Publication Year :
2023
Publisher :
Springer US, 2023.

Abstract

The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subsequent monitoring of their traffic toward acceptor compartments. Here we describe the RUSH system applied to cytotoxic T cells to characterize the biogenesis of lytic granules, using as a proof-of-concept granzyme B trafficking to this specialized compartment.

Details

ISBN :
978-1-07-163134-8
978-1-07-163135-5
ISSN :
10643745 and 19406029
ISBNs :
9781071631348 and 9781071631355
Database :
OpenAIRE
Journal :
The Immune Synapse ISBN: 9781071631348, Methods in Molecular Biology, Methods in Molecular Biology-The Immune Synapse
Accession number :
edsair.doi.dedup.....291a8706241fb7fdc6ed4a2cd68e60a1
Full Text :
https://doi.org/10.1007/978-1-0716-3135-5_27