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Proton-sensing G protein-coupled receptor mobilizes calcium in human synovial cells
- Source :
- American Journal of Physiology-Cell Physiology. 289:C601-C608
- Publication Year :
- 2005
- Publisher :
- American Physiological Society, 2005.
-
Abstract
- Lowered extracellular pH in a variety of tissues is associated with increased tissue destruction and initiation of inflammatory processes. Although the acid-sensing receptors described previously are ion channels, we describe a G protein-coupled proton-sensitive receptor that stimulates Ca2+ release from intracellular stores in a tumor-derived synoviocyte cell line (SW982) and in primary cultures of human synovial cells from patients with inflammatory arthropathies. We established a link between proton-dependent receptor activation and intracellular Ca2+ mobilization by demonstrating 1) dependence on the integrity of the intracellular Ca2+ store, 2) independence from extracellular Ca2+, and 3) proton-induced production of inositol phosphate and 4) by abolishing the effect with GTPase inhibitors. We propose that this G protein-coupled acid-sensing receptor linked to intracellular Ca2+ mobilization in synoviocytes can contribute to downstream inflammatory and cellular proliferative processes in synovial fibroblasts. The acid-sensing receptor has distinct characteristics as a metabotropic G protein-coupled receptor on human synoviocytes in this emerging new class of receptors.
- Subjects :
- Guanine
Patch-Clamp Techniques
Physiology
G protein
Receptors, Leukotriene B4
chemistry.chemical_element
Calcium
Calcium imaging
Cell Line, Tumor
Extracellular
Humans
Receptor
G protein-coupled receptor
Receptors, Purinergic P2
Ryanodine receptor
Arthritis
Synovial Membrane
Sarcoma
Cell Biology
Fibroblasts
Hydrogen-Ion Concentration
Cell biology
chemistry
Synovial Cell
Biochemistry
Protons
Extracellular Space
Acids
Subjects
Details
- ISSN :
- 15221563 and 03636143
- Volume :
- 289
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Cell Physiology
- Accession number :
- edsair.doi.dedup.....29121e7c67ff78dfe5a3b5772d072de4