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Plasticity of Migrating CD1b(+) and CD1b(-) Lymph Dendritic Cells in the Promotion of Th1, Th2 and Th17 in Response to Salmonella and Helminth Secretions
- Source :
- Plos One 11 (8), . (2013), PLoS ONE, PLoS ONE, Public Library of Science, 2013, 8 (11), ⟨10.1371/journal.pone.0079537⟩, PLoS ONE, Vol 8, Iss 11, p e79537 (2013)
- Publication Year :
- 2013
-
Abstract
- International audience; Dendritic cells (DCs) are pivotal in the development of specific T-cell responses to control pathogens, as they govern both the initiation and the polarization of adaptive immunity. To investigate the capacities of migrating DCs to respond to pathogens, we used physiologically generated lymph DCs (L-DCs). The flexible polarization of L-DCs was analysed in response to Salmonella or helminth secretions known to induce different T cell responses. Mature conventional CD1b(+) L-DCs showed a predisposition to promote pro-inflammatory (IL-6), pro-Th1 (IL-12p40) and anti-inflammatory (IL-10) responses which were amplified by Salmonella, and limited to only IL-6 induction by helminth secretions. The other major population of L-DCs did not express the CD1b molecule and displayed phenotypic features of immaturity compared to CD1b(+) L-DCs. Salmonella infection reduced the constitutive expression of TNF-alpha and IL-4 mRNA in CD1b(-) L-DCs, whereas this expression was not affected by helminth secretions. The cytokine response of T cells promoted by L-DCs was analysed in T cell subsets after co-culture with Salmonella or helminth secretion-driven CD1b(+) or CD1b(-) L-DCs. T cells preferentially expressed the IL-17 gene, and to a lesser extent the IFN-gamma and IL-10 genes, in response to Salmonella-driven CD1b(+) L-DCs, whereas a preferential IL-10, IFN-gamma and IL-17 gene expression was observed in response to Salmonella-driven CD1b(-) L-DCs. In contrast, a predominant IL-4 and IL-13 gene expression by CD4(+) and CD8(+) T cells was observed after stimulation of CD1b(+) and CD1b(-) L-DCs with helminth secretions. These results show that mature conventional CD1b(+) L-DCs maintain a flexible capacity to respond differently to pathogens, that the predisposition of CD1b(-) L-DCs to promote a Th2 response can be oriented towards other Th responses, and finally that the modulation of migrating L-DCs responses is controlled more by the pathogen encountered than the L-DC subsets.
- Subjects :
- Salmonella
[SDV]Life Sciences [q-bio]
lcsh:Medicine
medicine.disease_cause
immune response
Antigens, CD1
0302 clinical medicine
Cell Movement
Gene expression
Cytotoxic T cell
lcsh:Science
0303 health sciences
education.field_of_study
Multidisciplinary
biology
Salmonella enterica
hemic and immune systems
T-Lymphocytes, Helper-Inducer
Acquired immune system
medicine.anatomical_structure
Cytokines
Female
Research Article
delta t cell
infection
polarization
T cell
Population
réponse immunitaire
chemical and pharmacologic phenomena
polarisation
Microbiology
03 medical and health sciences
Th2 Cells
Helminths
medicine
Animals
education
030304 developmental biology
Sheep
lcsh:R
Dendritic Cells
Th1 Cells
biology.organism_classification
Th17 Cells
lcsh:Q
CD8
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- Plos One 11 (8), . (2013), PLoS ONE, PLoS ONE, Public Library of Science, 2013, 8 (11), ⟨10.1371/journal.pone.0079537⟩, PLoS ONE, Vol 8, Iss 11, p e79537 (2013)
- Accession number :
- edsair.doi.dedup.....290b3a905d25d8392603fd18268788b3