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The European antibody network's practical guide to finding and validating suitable antibodies for research
- Source :
- mAbs, Volume 8, Issue 1, Mabs, 8, 27-36, Mabs, 8, 1, pp. 27-36
- Publication Year :
- 2015
- Publisher :
- Taylor & Francis, 2015.
-
Abstract
- Contains fulltext : 171662.pdf (Publisher’s version ) (Open Access) Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody-based data fit for publication.
- Subjects :
- 0301 basic medicine
BLAST searches
CiteAb
EuroMAbNet
flow cytometry
gene silencing
HLDA workshops
immunohistochemistry
monoclonal antibodies
validation studies
western blotting
Biomedical Research
Databases, Factual
medicine.drug_class
media_common.quotation_subject
Immunology
Monoclonal antibody
Diagnostic tools
03 medical and health sciences
Antibodies monoclonal
Antibody activity
medicine
Immunology and Allergy
Animals
Humans
Quality (business)
media_common
Target antigen
Women's cancers Radboud Institute for Molecular Life Sciences [Radboudumc 17]
biology
business.industry
BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences
Antibodies, Monoclonal
Research opportunities
Data science
Biotechnology
Europe
030104 developmental biology
Perspective
biology.protein
Antibody
business
BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti
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Details
- Language :
- English
- ISSN :
- 19420870 and 19420862
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- mAbs
- Accession number :
- edsair.doi.dedup.....28f0f5986bbb29ed36cf912c7a6b2df5