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Varying Atmospheric CO2 Mediates the Cold-Induced CBF-Dependent Signaling Pathway and Freezing Tolerance in Arabidopsis
- Source :
- International Journal of Molecular Sciences, Vol 21, Iss 7616, p 7616 (2020), International Journal of Molecular Sciences, Volume 21, Issue 20
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Changes in the stomatal aperture in response to CO2 levels allow plants to manage water usage, optimize CO2 uptake and adjust to environmental stimuli. The current study reports that sub-ambient CO2 up-regulated the low temperature induction of the C-repeat Binding Factor (CBF)-dependent cold signaling pathway in Arabidopsis (Arabidopsis thaliana) and the opposite occurred in response to supra-ambient CO2. Accordingly, cold induction of various downstream cold-responsive genes was modified by CO2 treatments and expression changes were either partially or fully CBF-dependent. Changes in electrolyte leakage during freezing tests were correlated with CO2&prime<br />s effects on CBF expression. Cold treatments were also performed on Arabidopsis mutants with altered stomatal responses to CO2, i.e., high leaf temperature 1-2 (ht1-2, CO2 hypersensitive) and &beta<br />carbonic anhydrase 1 and 4 (ca1ca4, CO2 insensitive). The cold-induced expression of CBF and downstream CBF target genes plus freezing tolerance of ht1-2 was consistently less than that for Col-0, suggesting that HT1 is a positive modulator of cold signaling. The ca1ca4 mutant had diminished CBF expression during cold treatment but the downstream expression of cold-responsive genes was either similar to or greater than that of Col-0. This finding suggested that &beta<br />CA1/4 modulates the expression of certain cold-responsive genes in a CBF-independent manner. Stomatal conductance measurements demonstrated that low temperatures overrode low CO2-induced stomatal opening and this process was delayed in the cold tolerant mutant, ca1ca4, compared to the cold sensitive mutant, ht1-2. The similar stomatal responses were evident from freezing tolerant line, Ox-CBF, overexpression of CBF3, compared to wild-type ecotype Ws-2. Together, these results indicate that CO2 signaling in stomata and CBF-mediated cold signaling work coordinately in Arabidopsis to manage abiotic stress.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Stomatal conductance
abiotic stress
atmospheric CO2
Acclimatization
cold-responsive gene
Mutant
Arabidopsis
01 natural sciences
Catalysis
Article
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
stomatal regulation
Freezing
Arabidopsis thaliana
Physical and Theoretical Chemistry
C-repeat binding factor
Molecular Biology
Gene
lcsh:QH301-705.5
Spectroscopy
Freezing tolerance
biology
Chemistry
Abiotic stress
Arabidopsis Proteins
Atmosphere
Cold-Shock Response
Organic Chemistry
carbon dioxide
General Medicine
biology.organism_classification
freezing tolerance
Computer Science Applications
Cell biology
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Signal transduction
010606 plant biology & botany
Signal Transduction
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 21
- Issue :
- 7616
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....28e3ec516856cb71d2bf27714cc7cb07