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Plexin-B2 and Plexin-D1 in dendritic cells: expression and IL-12/IL-23p40 production
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 8, p e43333 (2012)
- Publication Year :
- 2011
-
Abstract
- Plexins are a family of genes (A,B,C, and D) that are expressed in many organ systems. Plexins expressed in the immune system have been implicated in cell movement and cell-cell interaction during the course of an immune response. In this study, the expression pattern of Plexin-B2 and Plexin-D1 in dendritic cells (DCs), which are central in immune activation, was investigated. Plexin-B2 and Plexin-D1 are reciprocally expressed in myeloid and plasmacytoid DC populations. Plasmacytoid DCs have high Plexin-B2 but low Plexin-D1, while the opposite is true of myeloid DCs. Expression of Plexin-B2 and Plexin-D1 is modulated upon activation of DCs by TLR ligands, TNFα, and anti-CD40, again in a reciprocal fashion. Semaphorin3E, a ligand for Plexin-D1 and Plexin-B2, is expressed by T cells, and interestingly, is dramatically higher on Th2 cells and on DCs. The expression of Plexins and their ligands on DCs and T cells suggest functional relevance. To explore this, we utilized chimeric mice lacking Plxnb2 or Plxnd1. Absence of Plexin-B2 and Plexin-D1 on DCs did not affect the ability of these cells to upregulate costimulatory molecules or the ability of these cells to activate antigen specific T cells. Additionally, Plexin-B2 and Plexin-D1 were dispensable for chemokine-directed in-vitro migration of DCs towards key DC chemokines, CXCL12 and CCL19. However, the absence of either Plexin-B2 or Plexin-D1 on DCs leads to constitutive expression of IL-12/IL-23p40. This is the first report to show an association between Plexin-B2 and Plexin-D1 with the negative regulation of IL-12/IL-23p40 in DCs. This work also shows the presence of Plexin-B2 and Plexin-D1 on mouse DC subpopulations, and indicates that these two proteins play a role in IL-12/IL-23p40 production that is likely to impact the immune response.
- Subjects :
- Chemokine
Myeloid
lcsh:Medicine
Gene Expression
Antigen Processing and Recognition
Interleukin-23
Biochemistry
Mice
0302 clinical medicine
Bone Marrow
Molecular Cell Biology
lcsh:Science
Cells, Cultured
0303 health sciences
Multidisciplinary
Membrane Glycoproteins
Reverse Transcriptase Polymerase Chain Reaction
Intracellular Signaling Peptides and Proteins
Flow Cytometry
Interleukin-12
Innate Immunity
Cell biology
medicine.anatomical_structure
embryonic structures
Interleukin 12
Cytokines
Research Article
animal structures
Immune Cells
Immunology
Antigen-Presenting Cells
Enzyme-Linked Immunosorbent Assay
Nerve Tissue Proteins
Biology
Immune Activation
03 medical and health sciences
Immune system
medicine
Animals
Protein Interactions
Immunity to Infections
B cell
030304 developmental biology
Inflammation
Follicular dendritic cells
CCL19
Plexin
lcsh:R
Immunity
Proteins
Immunoregulation
Dendritic Cells
Mice, Inbred C57BL
Immune System
biology.protein
lcsh:Q
030215 immunology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....28de3aa55abfd33d6d2b30634b4c4adc