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Adenoviral and Adeno-Associated Viral Vectors-Mediated Neuronal Gene Transfer to Cardiovascular Control Regions of the Rat Brain
- Source :
- International Journal of Medical Sciences
- Publication Year :
- 2013
- Publisher :
- Ivyspring International Publisher, 2013.
-
Abstract
- Viral vectors have been utilized extensively to introduce genetic material into the central nervous system. In order to investigate gene functions in cardiovascular control regions of rat brain, we applied WPRE (woodchuck hepatitis virus post-transcriptional regulatory element) enhanced-adenoviral (Ad) and adeno-assoicated virus (AAV) type 2 vectors to mediate neuronal gene delivery to the paraventricular nucleus of the hypothalamus, the nucleus tractus solitarius and the rostral ventrolateral medulla, three important cardiovascular control regions known to express renin-angiotensin system (RAS) genes. Ad or AAV2 harboring an enhanced green fluorescent protein (EGFP) reporter gene or the angiotensin type 2 receptor gene were microinjected into these brain regions in adult rats. Our results demonstrated that both AAV2 and Ad vectors elicited long-term neuronal transduction in these regions. Interestingly, we found that the WPRE caused expression of GFP driven by the synapsin1 promoter in pure glial cultures or co-cultures of neurons and glia derived from rat hypothalamus and brainstem. However, in rat paraventricular nucleus WPRE did not cause expression of GFP in glia. This demonstrates the potential use of these vectors in studies of physiological functions of certain genes in the cardiovascular control regions of the brain.
- Subjects :
- Male
cardiovascular control regions of brain
viruses
Genetic Vectors
Green Fluorescent Proteins
Gene delivery
Biology
Regulatory Sequences, Nucleic Acid
Cardiovascular System
Viral vector
Green fluorescent protein
Adenoviridae
Renin-Angiotensin System
03 medical and health sciences
Transduction (genetics)
0302 clinical medicine
Animals
gene transfer
Promoter Regions, Genetic
030304 developmental biology
Neurons
0303 health sciences
Reporter gene
Brain Mapping
Woodchuck hepatitis virus
Gene Transfer Techniques
Brain
General Medicine
Rostral ventrolateral medulla
biology.organism_classification
Molecular biology
neuron
Rats
nervous system
Hypothalamus
adenoviral vector
adeno-associated viral vector
030217 neurology & neurosurgery
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 14491907
- Volume :
- 10
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- International Journal of Medical Sciences
- Accession number :
- edsair.doi.dedup.....28dc3cc908a7cfdc3a4ce30c1c40c37a