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Superoxide dismutase and leupeptin prevent delayed reperfusion injury in the rat small intestine during burn shock
- Source :
- The Journal of burn carerehabilitation. 16(2 Pt 1)
- Publication Year :
- 1995
-
Abstract
- Delayed fluid resuscitation during burn shock is thought to compromise the integrity of gut mucosa and allow enteric bacteria to cross the luminal wall and infect other sterile organ systems. Superoxide dismutase, a free-oxygen radical scavenger; leupeptin, a protease inhibitor; and verapamil, a calcium channel blocker, were studied to evaluate their efficacy in maintaining cellular integrity in the gut of thermally burned rats whose fluid resuscitation had been delayed. Fifty male rats weighting 280 to 320 gm were given a full-thickness scald burn covering 50% total body surface area. Ten received early fluid resuscitation beginning half an hour after burn, and 40 received fluid resuscitation delayed by 6 hours. Those receiving delayed resuscitation were given superoxide dismutase (n = 10), leupeptin (n = 10), verapamil (n = 10), or a placebo of normal saline solution (n = 10) at the time of fluid resuscitation. Ileal mucosa samples were harvested, and adenosine triphosphate, diphosphate, and monophosphate were measured. Adenosine triphosphate, total nucleotides, and energy charge potential were significantly lower in the placebo group without therapy compared with those of the early resuscitation group. Superoxide dismutase and leupeptin therapy prevented this drop in cellular energy. Total water content was significantly increased in the placebo group compared with that of the early resuscitation group; superoxide dismutase was able to prevent this increase. Data indicate that intestinal reperfusion injury in burned rats can be effectively modulated with superoxide dismutase or leupeptin therapy.
- Subjects :
- Male
Resuscitation
Pathology
medicine.medical_specialty
Time Factors
medicine.drug_class
Leupeptins
medicine.medical_treatment
Calcium channel blocker
Pharmacology
Superoxide dismutase
Rats, Sprague-Dawley
chemistry.chemical_compound
Adenosine Triphosphate
Intestine, Small
medicine
Animals
Intestinal Mucosa
Saline
General Nursing
biology
business.industry
Superoxide Dismutase
Rehabilitation
Leupeptin
Shock
medicine.disease
Adenosine Monophosphate
Rats
Adenosine Diphosphate
chemistry
Verapamil
Shock (circulatory)
Reperfusion Injury
General Health Professions
Emergency Medicine
biology.protein
Fluid Therapy
Surgery
medicine.symptom
business
Burns
Reperfusion injury
medicine.drug
Subjects
Details
- ISSN :
- 02738481
- Volume :
- 16
- Issue :
- 2 Pt 1
- Database :
- OpenAIRE
- Journal :
- The Journal of burn carerehabilitation
- Accession number :
- edsair.doi.dedup.....2891dc826549e53a9a2c97301863f402