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Members of the miR-290 cluster modulate in vitro differentiation of mouse embryonic stem cells
- Source :
- Differentiation. 78:69-78
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- We report the biological effects of miR-290 cluster via gain-of-function or loss-of-function experiments in mouse embryonic stem cells (ESCs) cultured under differentiation conditions. Under these conditions we found that overexpression of miR-290 cluster in ESCs cannot prevent downregulation of Oct-4, but inhibition results in earlier downregulation of Oct-4 compared with the negative control. In consistence with previous findings that report ectopic expression of Brachyury during gastrulation in Argonaute-2 KO mice due to impaired miRNA function, we show that miR-290 cluster regulates negatively differentiation of ESCs towards mesodermal and germ cell lineage. These results suggest that although incapable to maintain pluripotent state alone, miR-290 cluster inhibits ESC differentiation and it is involved in the pathways controlling mesoderm and primordial germ cell differentiation. Finally, we provide proofs that members of this cluster target Dkk-1 gene, a Wnt pathway inhibitor, and affect this pathway, which can partially explain why miR-290 cluster favours pluripotency against differentiation.
- Subjects :
- Pluripotent Stem Cells
Cancer Research
Chromosomal Proteins, Non-Histone
Cellular differentiation
Embryoid body
Biology
Mesoderm
Mice
03 medical and health sciences
0302 clinical medicine
medicine
Animals
RNA, Messenger
Luciferases
Molecular Biology
Cells, Cultured
Embryonic Stem Cells
030304 developmental biology
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Wnt signaling pathway
Cell Differentiation
Cell Biology
Molecular biology
Embryonic stem cell
Cell biology
Repressor Proteins
Endothelial stem cell
MicroRNAs
Germ Cells
medicine.anatomical_structure
embryonic structures
Intercellular Signaling Peptides and Proteins
Germ line development
Stem cell
Octamer Transcription Factor-3
030217 neurology & neurosurgery
Germ cell
Developmental Biology
Subjects
Details
- ISSN :
- 03014681
- Volume :
- 78
- Database :
- OpenAIRE
- Journal :
- Differentiation
- Accession number :
- edsair.doi.dedup.....287a0f187511bddba0fa77c6f0cd1f9e