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Correction to: Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
- Source :
- Virology Journal, Virology Journal, BioMed Central, 2019, 16 (1), ⟨10.1186/s12985-019-1118-8⟩, Virology Journal, 2019, 16 (1), ⟨10.1186/s12985-019-1118-8⟩, Virology Journal, Vol 16, Iss 1, Pp 1-1 (2019)
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies. Electronic supplementary material The online version of this article (10.1186/s12985-018-1105-5) contains supplementary material, which is available to authorized users.
- Subjects :
- 0303 health sciences
biology
viruses
[SDV]Life Sciences [q-bio]
Short Report
biology.organism_classification
Virology
3. Good health
Zika virus
lcsh:Infectious and parasitic diseases
03 medical and health sciences
0302 clinical medicine
Infectious Diseases
030220 oncology & carcinogenesis
Seroprevalence
lcsh:RC109-216
Virus neutralization test
ComputingMilieux_MISCELLANEOUS
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
030304 developmental biology
Seroepidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 1743422X
- Database :
- OpenAIRE
- Journal :
- Virology Journal, Virology Journal, BioMed Central, 2019, 16 (1), ⟨10.1186/s12985-019-1118-8⟩, Virology Journal, 2019, 16 (1), ⟨10.1186/s12985-019-1118-8⟩, Virology Journal, Vol 16, Iss 1, Pp 1-1 (2019)
- Accession number :
- edsair.doi.dedup.....2873d04fff559a0c77af4246e2b7af4c
- Full Text :
- https://doi.org/10.1186/s12985-019-1118-8⟩