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Correction to: Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

Authors :
Boris Pastorino
Bouba Gake
Paola Mariela Saba Villarroel
Pierre Gallian
Elif Nurtop
Jan Felix Drexler
Yelin Roca
Stéphane Priet
Xavier de Lamballerie
Christophe N. Peyrefitte
Audrey Dubot-Pérès
Gilda Grard
Laetitia Ninove
Unité des Virus Emergents (UVE)
Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Institute of Virology
University of Bonn Medical Centre
Centro Nacional de Enfermedades Tropicales [Santa Cruz de la Sierra, Bolivia] (CENETROP)
Centre Pasteur du Cameroun
Réseau International des Instituts Pasteur (RIIP)
Nuffield Department of Clinical Medicine [Oxford]
University of Oxford [Oxford]
Centre National de Référence des Arbovirus [Marseille]
Institut de Recherche Biomédicale des Armées [Antenne Marseille] (IRBA)-Unité d'Arbovirologie [Marseille]
Hôpital d'Instruction des Armées Laveran
Service de Santé des Armées-Service de Santé des Armées-Hôpital d'Instruction des Armées Laveran
Service de Santé des Armées-Service de Santé des Armées
Emerging Pathogens Laboratory
Fondation Mérieux
Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille)
Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)
University of Oxford
Source :
Virology Journal, Virology Journal, BioMed Central, 2019, 16 (1), ⟨10.1186/s12985-019-1118-8⟩, Virology Journal, 2019, 16 (1), ⟨10.1186/s12985-019-1118-8⟩, Virology Journal, Vol 16, Iss 1, Pp 1-1 (2019)
Publication Year :
2019
Publisher :
HAL CCSD, 2019.

Abstract

Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies. Electronic supplementary material The online version of this article (10.1186/s12985-018-1105-5) contains supplementary material, which is available to authorized users.

Details

Language :
English
ISSN :
1743422X
Database :
OpenAIRE
Journal :
Virology Journal, Virology Journal, BioMed Central, 2019, 16 (1), ⟨10.1186/s12985-019-1118-8⟩, Virology Journal, 2019, 16 (1), ⟨10.1186/s12985-019-1118-8⟩, Virology Journal, Vol 16, Iss 1, Pp 1-1 (2019)
Accession number :
edsair.doi.dedup.....2873d04fff559a0c77af4246e2b7af4c
Full Text :
https://doi.org/10.1186/s12985-019-1118-8⟩