Back to Search
Start Over
Evaluation of miRNA-21 and miRNA Let-7 as Prognostic Markers in Patients With Breast Cancer
- Source :
- Clinical breast cancer. 18(4)
- Publication Year :
- 2017
-
Abstract
- Breast cancer remains one of the top threats to women's health. The current lack of tumor markers with desirable sensitivity and specificity is a major obstacle toward the future management of breast cancer. Many studies are directed to reveal the diagnostic and prognostic potentials of circulating miRNAs in breast cancer. In this study, we attempt to evaluate the feasibility and clinical utility of circulating miRNA-21 and let-7 as prognostic biomarkers for breast cancer.Real-time quantitative polymerase chain reaction technique was used. Levels of miRNA-21 and let-7 expression were determined in sera from 125 participants representing 3 different groups. With fold-change analysis, the expression of miRNA-21 and let-7 in the decided groups were assessed.Patients with breast cancer showed significantly higher expression of miRNA-21 compared with controls and other participants with benign breast lesions (P .001). The mean expression levels of serum miRNA-21 was 3.27 ± 2.10-fold in patients with breast cancer. The expression of miRNA let-7 was significantly decreased in patients with breast cancer (2.45 ± 2.20-fold) than the control group and the benign breast lesions group (5.27 ± 3.30-fold and 6.22 ± 4.90-fold, respectively; P .001). Levels of miRNA let-7 expression negatively correlated with development of metastases in patients with breast cancer (P .001).Our study establishes the association between altered levels of miRNA let-7 and metastases risk in patients with breast cancer, implying a role of miRNA let-7 in disease progression and prognosis.
- Subjects :
- 0301 basic medicine
Oncology
Circulating mirnas
Adult
Cancer Research
medicine.medical_specialty
Breast Neoplasms
03 medical and health sciences
0302 clinical medicine
Breast cancer
Internal medicine
microRNA
medicine
Biomarkers, Tumor
Humans
In patient
Neoplasm Metastasis
skin and connective tissue diseases
business.industry
MiRNA Let-7
Disease progression
Middle Aged
medicine.disease
Prognosis
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
Disease Progression
Female
business
Subjects
Details
- ISSN :
- 19380666
- Volume :
- 18
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Clinical breast cancer
- Accession number :
- edsair.doi.dedup.....286f080e2cd4382c4a3b30bea5682479