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Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae
- Source :
- Acta Crystallogr F Struct Biol Commun
- Publication Year :
- 2021
-
Abstract
- GldL is an inner-membrane protein that is essential for the function of the type IX secretion system (T9SS) in Flavobacterium johnsoniae. The complex that it forms with GldM is supposed to act as a new rotary motor involved in the gliding motility of the bacterium. In the context of structural studies of GldL to gain information on the assembly and function of the T9SS, two camelid nanobodies were selected, produced and purified. Their interaction with the cytoplasmic domain of GldL was characterized and their crystal structures were solved. These nanobodies will be used as crystallization chaperones to help in the crystallization of the cytoplasmic domain of GldL and could also help to solve the structure of the complex using molecular replacement.
- Subjects :
- Models, Molecular
Camelus
Gliding motility
Biophysics
Context (language use)
Crystal structure
Crystallography, X-Ray
Biochemistry
Flavobacterium
Research Communications
03 medical and health sciences
Bacterial Proteins
Protein Domains
Structural Biology
Genetics
Animals
Scattering, Radiation
Molecular replacement
Secretion
Flavobacterium johnsoniae
Bacterial Secretion Systems
030304 developmental biology
0303 health sciences
030306 microbiology
Chemistry
Single-Domain Antibodies
Condensed Matter Physics
Kinetics
Cytoplasm
Thermodynamics
Protein Multimerization
Function (biology)
Subjects
Details
- ISSN :
- 2053230X
- Volume :
- 77
- Issue :
- Pt 6
- Database :
- OpenAIRE
- Journal :
- Acta crystallographica. Section F, Structural biology communications
- Accession number :
- edsair.doi.dedup.....286ba0dd8fe9b28e4c94af4d6b855795