Back to Search
Start Over
Escape from R-peptide deletion in a γ-retrovirus
- Source :
- Virology. 418:85-92
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- The R peptide in the cytoplasmic tail (C-tail) of γ-retroviral envelope proteins (Env) prevents membrane fusion before budding. To analyse its role in the formation of replication competent, infectious particles, we developed chimeric murine leukaemia viruses (MLV) with unmodified or R-peptide deleted Env proteins of the gibbon ape leukaemia virus (GaLV). While titres of these viruses were unaffected, R-peptide deficiency led to strongly impaired spreading. Most remarkably, we isolated an escape mutant which had restored an open reading frame for a C-terminal extension of the truncated C-tail. A reconstituted virus encoding this escape C-tail replicated in cell culture. In contrast to R-peptide deficient Env, particle incorporation of the escape Env was effective due to an enhanced protein expression and restored intracellular co-localisation with Gag proteins. Our data demonstrate that the R peptide not only regulates membrane fusion but also mediates efficient Env protein particle incorporation in γ-retrovirus infected cells.
- Subjects :
- Gene Expression Regulation, Viral
Evolution
viruses
Mutant
MLV
Biology
Virus Replication
Envelope protein
SH3 domain
Virus
Cell Line
Viral Proteins
Retrovirus
Virology
Animals
Humans
chemistry.chemical_classification
R peptide
Virus Assembly
Lipid bilayer fusion
biology.organism_classification
Molecular biology
Open reading frame
Retroviridae
chemistry
Cell culture
Glycoprotein
Oligopeptides
Gene Deletion
Reassortant Viruses
GaLV
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 418
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....2867017ce02f94030583f12f52263046