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Cell-free microRNA-1246 in different body fluids as a diagnostic biomarker for esophageal squamous cell carcinoma
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 3, p e0248016 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science, 2021.
-
Abstract
- Esophageal squamous cell carcinoma is a malignant tumor with unfavorable prognosis. In this study, we investigated the usefulness of microRNA (miR)-1246 detection in various body fluids as a biomarker for this disease. A total of 72 patients with esophageal squamous cell carcinoma were enrolled, and their blood, urine, and saliva samples were collected prior to treatment. Reverse transcription–polymerase chain reaction of miR-1246 was performed, and pre- and postoperative and intraday fluctuations in its expression were examined. The expression of miR-1246 in the blood and urine was significantly higher in the patients with esophageal squamous cell carcinoma than in 50 healthy control subjects. Receiver operating characteristic curves showed that the area under the curve values were 0.91 (sensitivity 91.7%, specificity 76.0%), 0.82 (sensitivity 90.3%, specificity 62.0%), and 0.80 (sensitivity 83.3%, specificity 66.0%) in the serum, urine, and saliva, respectively. A relatively high diagnostic performance of miR-1246 was observed in all samples, which was better than that of the existing biomarkers squamous cell carcinoma antigen, carcinoembryonic antigen, and cytokeratin 19 fragment. No clear correlation was observed in the levels of miR-1246 expression among the three body fluids. Postoperatively, serum samples displayed significantly decreased miR-1246 levels. Although not significant, changes in the miR-1246 levels were observed at all collection times, with large fluctuations in the saliva. Meanwhile, serum miR-1246 expression was found to be associated with the disease prognosis. The results indicate that the levels of miR-1246 in the urine, saliva, and serum are a useful biomarker for esophageal squamous cell carcinoma and support the use of urine samples instead of blood samples for noninvasive diagnosis.
- Subjects :
- Male
Saliva
Esophageal Neoplasms
Physiology
Urine
Gastroenterology
Esophageal squamous cell carcinoma
Biochemistry
Lung and Intrathoracic Tumors
Carcinoembryonic antigen
Japan
Medicine and Health Sciences
Aged, 80 and over
Multidisciplinary
biology
Area under the curve
Middle Aged
Prognosis
Body Fluids
Oncology
Carcinoma, Squamous Cell
Medicine
Biomarker (medicine)
Female
Esophageal Squamous Cell Carcinoma
Anatomy
Research Article
medicine.medical_specialty
Science
Disease-Free Survival
Malignant Tumors
Internal medicine
microRNA
Gastrointestinal Tumors
medicine
Biomarkers, Tumor
Humans
Squamous Cell Carcinoma
Circulating MicroRNA
Aged
Receiver operating characteristic
business.industry
Gene Expression Profiling
Carcinoma
Biology and Life Sciences
Cancers and Neoplasms
Non-Small Cell Lung Cancer
Gastric Cancer
MicroRNAs
ROC Curve
biology.protein
business
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....284e81191074c5391cd6960e0fc66933