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Angiotensin-II-Induced Expression of Laminin Complex and Laminin A-Chain-Related Transcripts in Vascular Smooth Muscle Cells
- Source :
- Journal of Vascular Research. 31:163-172
- Publication Year :
- 1994
- Publisher :
- S. Karger AG, 1994.
-
Abstract
- Laminin, a major structural glycoprotein complex of basement membranes has been found to be modulated by angiotensin II in vitro and in vivo. In cultures of aortic organoids and vascular smooth muscle cells, expression of laminin was stimulated by exogenous vasoconstrictor peptide. Stimulation of laminin protein and mRNA expression was observed for both laminin B1/B2-chains and an unknown laminin heavy chain. Compared with PYS-2 cells, a mouse teratocarcinoma cell line which constitutively expresses a 10-kb mRNA transcript for 'classical' laminin A-chain, cultured vascular smooth muscle cells (VSMC) did not express a corresponding mRNA. However, cultured VSMC were found to express laminin A-chain-related mRNAs of approximately 1.8 kb and approximately 3.8 kb, respectively. The 1.8-kb species of transcript was expressed in a constitutive manner, whereas the 3.8-kb mRNA was found to be regulated by angiotensin II. Laminin complexes secreted by cultured cells contained a approximately 300 kD heavy chain which did not immunoreact with immunoreagents raised against either the classical laminin complex secreted by EHS tumor cells or the merosin heavy chain. The putative A-chain analogue possibly represents a new form of a tissue-specific laminin heavy chain, distinct from the A- and M-chains thus far described. Translation products encoded by the A-chain-related transcripts of cultured smooth muscle cells could not be specified using currently available antibodies. The putative protein(s) is speculated to contain the biological features of the N-terminus of the laminin A-chain, namely self-assembly and association with collagen type IV.
- Subjects :
- Male
Teratocarcinoma
Vascular smooth muscle
Transcription, Genetic
Physiology
Blotting, Western
Muscle, Smooth, Vascular
Mice
Laminin
Rats, Inbred SHR
Tumor Cells, Cultured
Protein biosynthesis
Animals
Myocyte
RNA, Messenger
Laminin complex
Aorta
Cells, Cultured
Messenger RNA
Dose-Response Relationship, Drug
biology
Chemistry
Blotting, Northern
Immunohistochemistry
Precipitin Tests
Molecular biology
Angiotensin II
Rats
Organoids
Gene Expression Regulation
Cell culture
Protein Biosynthesis
biology.protein
Endothelium, Vascular
DNA Probes
Cardiology and Cardiovascular Medicine
Subjects
Details
- ISSN :
- 14230135 and 10181172
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Journal of Vascular Research
- Accession number :
- edsair.doi.dedup.....2824223e51dceca016ee958a7abc6e7b