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Chryseobacterium phosphatilyticum sp. nov., a phosphate-solubilizing endophyte isolated from cucumber (Cucumis sativus L.) root
- Source :
- International journal of systematic and evolutionary microbiology. 69(3)
- Publication Year :
- 2018
-
Abstract
- A bacterial strain, designated as ISE14T, with Gram-stain-negative and non-motile rod-shaped cells, was isolated from the root of a cucumber plant collected in a field in Iksan, Republic of Korea and was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISE14T represented a member of the genus Chryseobacterium and was closely related to Chryseobacterium viscerum 687B-08T (16S rRNA gene sequence similarity of 98.50 %), Chryseobacterium lactis NCTC 11390T (98.49 %), Chryseobacterium ureilyticum F-Fue-04IIIaaaaT (98.49 %) and Chryseobacterium oncorhynchi 701B-08T (98.04 %). Average nucleotide identity values between genome sequences of strain ISE14T and the closely related species ranged from 81.44 to 83.15 %, which were lower than the threshold of 95 % (corresponding to a DNA–DNA hybridization value of 70 %). The DNA G+C content of strain ISE14T was 36.3 mol%. The dominant fatty acids were iso-C15 : 0, summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids and eight unidentified lipids; the predominant respiratory quinone was MK-6. On the basis of the evidence presented in this study, strain ISE14T can be distinguished from closely related species belonging to the genus Chryseobacterium . Thus, strain ISE14T is a novel species of the genus Chryseobacterium , for which the name Chryseobacterium phosphatilyticum sp. nov. is proposed. The type strain is ISE14T (=KACC 19820T=JCM 32876T).
- Subjects :
- 0106 biological sciences
0301 basic medicine
DNA, Bacterial
Chryseobacterium ureilyticum
Chryseobacterium
010603 evolutionary biology
01 natural sciences
Microbiology
Chryseobacterium viscerum
Plant Roots
Chryseobacterium lactis
Phosphates
03 medical and health sciences
RNA, Ribosomal, 16S
Chryseobacterium oncorhynchi
Republic of Korea
Endophytes
Ecology, Evolution, Behavior and Systematics
Phylogeny
Base Composition
Strain (chemistry)
biology
Phosphatidylethanolamines
Fatty Acids
Nucleic Acid Hybridization
Vitamin K 2
General Medicine
Sequence Analysis, DNA
16S ribosomal RNA
biology.organism_classification
Bacterial Typing Techniques
030104 developmental biology
Cucumis sativus
Cucumis
Subjects
Details
- ISSN :
- 14665034
- Volume :
- 69
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- International journal of systematic and evolutionary microbiology
- Accession number :
- edsair.doi.dedup.....280f34322e15b5d9fef0b76426a93d06