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Influence of Polyelectrolyte Multilayer Films on the ICAM-1 Expression of Endothelial Cells
- Source :
- Cell Biochemistry and Biophysics. 44:223-232
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- Recently, the use of polyelectrolyte films has been suggested as a new versatile technique of surface modification aimed at tissue engineering. In the present study, we evaluated the expression of intercellular adhesion molecule (ICAM)-1 of endothelial cells (ECs) seeded on two types of polyelectrolyte multilayer films either terminated by poly(D-lysine) (PDL) or poly(allylamine hydrochloride) (PAH). This work showed that chemical stimulations with tumor necrosis factor (TNF)-alpha induced the ICAM-1 expression of ECs differently depending largely on the film architecture employed. Compared with PAH-ending films, the PDL-ending ones upregulated the ICAM-1 expression of the ECs after a prolonged exposition to TNF-alpha, rendering this film type less favorable in tissue engineering. Cytochalasin D (an F-actin disrupting agent) showed the involvement of the cytoskeleton in the upregulation of ICAM-1 for cells deposited on films terminated by PDL. The PAH-ending films did not perturb the ICAM-1 expression of ECs and might thus enhance the seeding of ECs in vascular engineering.
- Subjects :
- Cytochalasin D
Surface Properties
Biophysics
Biochemistry
Allylamine
chemistry.chemical_compound
Tissue engineering
Polyamines
Humans
Cytoskeleton
Cells, Cultured
ICAM-1
Tissue Engineering
Tumor Necrosis Factor-alpha
Chemistry
Cell Biology
General Medicine
Intercellular Adhesion Molecule-1
Intercellular adhesion molecule
Polyelectrolytes
Surface modification
Tumor necrosis factor alpha
Endothelium, Vascular
Stress, Mechanical
Subjects
Details
- ISSN :
- 10859195
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Cell Biochemistry and Biophysics
- Accession number :
- edsair.doi.dedup.....280ac2172114088c60d3044377158c51