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Endostar, a recombined humanized endostatin, inhibits lymphangiogenesis and lymphatic metastasis of Lewis lung carcinoma xenograft in mice
- Source :
- The Thoracic and cardiovascular surgeon. 59(3)
- Publication Year :
- 2011
-
Abstract
- Objective The aim of this study was to investigate the effects of Endostar, a recombined humanized endostatin, on lymphatic tumor growth, lymphangiogenesis and lymphatic metastasis of Lewis lung carcinoma (LLC) xenograft in mice. Methods Lewis lung carcinoma xenografts were established in C57BL/6 mice by intravenous injection of 1 × 10(6) cells. Then the mice were assigned to two groups: the control group received caudal vein injections of 0.2 ml of 0.9% sodium chloride for 15 days, and the treatment group received 500 µg Endostar daily. Six weeks after LLC cell injection, the mice were sacrificed, and tumor multiplicity and tumor sizes were recorded. The expression of vascular endothelial growth factor C (VEGF-C) and podoplanin were observed by immunohistochemical staining. Results Tumor numbers and sizes in the control group were significantly higher than those of the treatment group. The microlymphatic vessel density (MLVD) was 5.67 ± 1.57 in the treatment group, which was markedly lower than in the control mice (7.78 ± 1.56). Two lymph node metastases were observed in the treatment group, and eight in the control group. Lymphatic metastases were more frequent in the control group than in the treatment group. Expression of VEGF-C in the control group was significantly higher than that in the treatment group. Conclusion Endostar significantly inhibits the lymphatic tumor growth, lymphangiogenesis and lymphatic metastasis of Lewis lung carcinoma xenografts, and its inhibitory effect is due to its ability to partially regulate the tumor expression of VEGF-C.
- Subjects :
- Pulmonary and Respiratory Medicine
Transplantation, Heterologous
Vascular Endothelial Growth Factor C
Carcinoma, Lewis Lung
Mice
Carcinoma
Medicine
Animals
Lymphangiogenesis
Lymph node
business.industry
Lewis lung carcinoma
medicine.disease
Endostatins
Transplantation
Gene Expression Regulation, Neoplastic
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Lymphatic system
Vascular endothelial growth factor C
Lymphatic Metastasis
Immunology
Cancer research
Surgery
Endostatin
Cardiology and Cardiovascular Medicine
business
Subjects
Details
- ISSN :
- 14391902
- Volume :
- 59
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The Thoracic and cardiovascular surgeon
- Accession number :
- edsair.doi.dedup.....28090c14fdb36a1b210c51eb86eb734c