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Common Lung Microbiome Identified among Mechanically Ventilated Surgical Patients
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 11, p e0166313 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- The examination of the pulmonary microbiome in patients with non-chronic disease states has not been extensively examined. Traditional culture based screening methods are often unable to identify bacteria from bronchoalveolar lavage samples. The advancement of next-generation sequencing technologies allows for a culture-independent molecular based analysis to determine the microbial composition in the lung of this patient population. For this study, the Ion Torrent PGM system was used to assess the microbial complexity of culture negative bronchoalveolar lavage samples. A group of samples were identified that all displayed high diversity and similar relative abundance of bacteria. This group consisted of Hydrogenophaga, unclassified Bacteroidetes, Pedobacter, Thauera, and Acinetobacter. These bacteria may be representative of a common non-pathogenic pulmonary microbiome associated within this population of patients.
- Subjects :
- 0301 basic medicine
Male
Pulmonology
lcsh:Medicine
Artificial Gene Amplification and Extension
Biochemistry
Polymerase Chain Reaction
Bronchoalveolar Lavage
Database and Informatics Methods
Antibiotics
RNA, Ribosomal, 16S
Hydrogenophaga
Medicine and Health Sciences
lcsh:Science
Lung
Pedobacter
education.field_of_study
Multidisciplinary
biology
medicine.diagnostic_test
Antimicrobials
Microbiota
Drugs
Genomics
Biodiversity
Middle Aged
Nucleic acids
Ribosomal RNA
Medical Microbiology
Female
Sequence Analysis
Research Article
Adult
Lung microbiome
Cell biology
food.ingredient
Cellular structures and organelles
Adolescent
030106 microbiology
Population
Sequence Databases
Microbial Genomics
Research and Analysis Methods
Microbiology
03 medical and health sciences
Young Adult
food
Diagnostic Medicine
Microbial Control
medicine
Genetics
Humans
Microbiome
education
Non-coding RNA
Molecular Biology Techniques
Sequencing Techniques
Molecular Biology
Pharmacology
Bacteria
lcsh:R
Organisms
Biology and Life Sciences
Ion semiconductor sequencing
Pneumonia
Acinetobacter
biology.organism_classification
Respiration, Artificial
030104 developmental biology
Bronchoalveolar lavage
Biological Databases
RNA
lcsh:Q
Ribosomes
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....2805412727c00325ebd36c00d69b66ca