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STUDIES ON THE CAUSES OF BACTERIAL MASTITIES IN TWO DAIRY BOVINE FARMS IN NORTHEASTERN BULGARIA AND THEIR SENSITIVITY TO ANTIMICROBIAL PRODUCTS

Authors :
Mariyana Nikolova
Valentina Urumova
Mihni Liuzkanov
Sasho Sabev
Faculty of Veterinary medicine
Publication Year :
2021
Publisher :
Zenodo, 2021.

Abstract

The prevalence of clinical and subclinical mastitis in two dairy farms with 500 and 114 cows each was studied. The causative agents have been identified and their antimicrobial susceptibility has been determined. In farm A, 44 cows (8.8%) with mastitis were observed - 3 with clinical and 41 with subclinical forms. Pathogenic microflora was isolated from 35 cows (79.5%). Were identified 64 strains: 30 (46.9%) Streptococ-cus spp. and 34 (53.1%) Staphylococcus spp. All staphylococci and streptococci showed sensitivity to Amoxi-cillin/Clav. acid. All staphylococci also showed sensitivity to Cephalothin, Gentamicin and Rifampicin. In farm B, 26 cows (22.8%) with mastitis were diagnosed - 10 with clinical and 16 with subclinical forms. Pathogenic microflora was isolated from 19 cows (73.1%). Were identified 24 strains: 8 (33.3%) Streptococcus spp. and 16 (66.7%) Staphylococcus spp. All streptococci showed sensitivity to Amoxicillin, Amoxicillin/Clav. acid, Cephalothin, Erythromycin, Gentamicin, Tetracycline, Rifampicin and Enrofloxacin. Susceptibility to Amoxicillin/Clav. acid, Cefoxitin, Cephalothin, Lincomycin, Gentamicin, Tetracycline, Rifampicin and En-rofloxacin was found in all staphylococci.<br />{"references":["Abebe R., Hatiya H., Abera M., Megersa B., Asmare K. (2016). Bovine mastitis: prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia. BMC Vet. Res., 12(1), 270.","Bauer, A. W., W. M. Kirby, J. C. Cherris, M. Truck. (1966). Antibiotic susceptibility testing by a standardized single disk method. The Am. J. of Clin. Pathol. 45–4, 493–496.","Condas L. A. Z., De Buck J., Nobrega D. B., Carson D. A., Roy J. P., Keefe G. P., DeVries T. J., Middleton J. R., Dufour S., Barkema H. W. (2017). Distribution of non-aureus staphylococci species in udder quarters with low and high somatic cell count, and clinical mastitis. J. Dairy Sci., 100(7), 5613–5627","Costa G.M., Barros R.A, Custódio D.A.C., Pereira U.P., Figueiredo D.J., Silva N. (2013). Antibiotic resistance in Staphylococcus aureus isolated from mastitis in dairy cattle from Minas Gerais, Brazil. Inst. ArchivesBiológico, São Paulo, 80(3), 297–302.","European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters.Version 11.0, valid from 2021-01-01. http://www.eucast.org.","Gomes F., Saavedra M. J., Henriques M. (2016). Bovine mastitis disease/pathogenicity: evidence of the potential role of microbial biofilms. Pathogens and Disease, 74(3), 1–7.","Kalayu А.A, Woldetsadik D.A., Woldeamanuel Y., Wang S.-A., Gebreyes W.A., Teferi T. (2020). Burden and antimicrobial resistance of S. aureus in dairy farms in Mekelle, Northern Ethiopia. BMC Vet. Res., 16(1), 20.","Lopes M.A., Demeu F.A., Franco Neto A. (2012). Evaluation of the economic impact of mastitis. Arq. Inst. Biol., São Paulo ,79(4), 477–483.","Mesquita A.A., Rocha C.M.B.M., Bruhn F.R.P., Custódio D.A.C, Braz M.S., Pinto S.M., Silva D. B., Costa G.M. (2019). Staphylococcus aureus and Streptococcus agalactiae: prevalence, resistance to antimicrobials, and their relationship with the milk quality of dairy cattle herds in Minas Gerais state, Brazil. Pesq. Vet. Bras. 39(5), 308–316.","Otto M. (2013). Coagulase‐negative staphylococci as reservoirs of genes facilitating MRSA infec-tion. Staphylococcal commensal species such as Staphylococcus epidermidis are being recognized as important sources of genes promoting MRSA colonization and virulence. Bioessays, 35(1), 4–11.","Ramírez N. F., Keefe G., Dohoo I., Sánchez J., Arroyave O., Cerón J., Jaramillo M., Palacio L. G.(2014). Herd- and cow-level risk factors associated with subclinical mastitis in dairy farms from the High Plains of the northern Antioquia, Colombia. J. Dairy Sci., 97(7), 4141–4150","Ruegg P.L. (2012). New perspectives in udder health management. Vet. Clin. N. Am., Food Anim. Pract., 28(2), 149–163.","Sentitula, Yadav B. R., Kumar R. (2012). Incidence of Staphylococci and Streptococci During Win-ter in Mastitic Milk of Sahiwal Cow and Murrah Buffaloes. Indian J Microbiol., 52(2), 153–159.","Sztachańska M., Barański W., Janowski T., Pogorzelska J., Zduńczyk S. (2016). Prevalence and etiological agents of subclinical mastitis at the end of lactation in nine dairy herds in North-East Poland. Pol. J. Vet. Sci.,19(1), 119–124.","Yang F. L., Shen C., He B. X., Yang Y. Y., Gong D. C., Li X. S.(2015). The prevalence of heifer mastitis and its associated risk factors in Huanggang, Central China. Trop. Anim. Health Prod., 47(1), 87–92.","Zeryehun T., Abera G. (2017). Prevalence and bacterial isolates of mastitis in dairy farms in se-lected districts of Eastern Harrarghe Zone, Eastern Ethiopia. J. Vet. Med. 2017(2), 1–7"]}

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....27fc343f184d8c4eff532bcf76b0e542
Full Text :
https://doi.org/10.5281/zenodo.5733980