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Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits
- Source :
- Nature. 367:463-467
- Publication Year :
- 1994
- Publisher :
- Springer Science and Business Media LLC, 1994.
-
Abstract
- The amiloride-sensitive epithelial sodium channel constitutes the rate-limiting step for sodium reabsorption in epithelial cells that line the distal part of the renal tubule, the distal colon, the duct of several exocrine glands, and the lung. The activity of this channel is upregulated by vasopressin and aldosterone, hormones involved in the maintenance of sodium balance, blood volume and blood pressure. We have identified the primary structure of the alpha-subunit of the rat epithelial sodium channel by expression cloning in Xenopus laevis oocytes. An identical subunit has recently been reported. Here we identify two other subunits (beta and gamma) by functional complementation of the alpha-subunit of the rat epithelial Na+ channel. The ion-selective permeability, the gating properties and the pharmacological profile of the channel formed by coexpressing the three subunits in oocytes are similar to that of the native channel.
- Subjects :
- Epithelial sodium channel
Molecular Sequence Data
Epithelium
Permeability
Sodium Channels
Cell Line
Membrane Potentials
Amiloride
Xenopus laevis
chemistry.chemical_compound
Benzamil
medicine
Animals
Amino Acid Sequence
Cells, Cultured
Multidisciplinary
Aldosterone
Renal sodium reabsorption
Sodium channel
Recombinant Proteins
Epithelial sodium channel blocker
Rats
Liddle Syndrome
Cell biology
chemistry
Oocytes
Ion Channel Gating
medicine.drug
Subjects
Details
- ISSN :
- 14764687 and 00280836
- Volume :
- 367
- Database :
- OpenAIRE
- Journal :
- Nature
- Accession number :
- edsair.doi.dedup.....27efd0153e6f53bfc0e72814645d6893