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Fluoro-Jade, but not Fluoro-Jade B, stains non-degenerating cells in brain and retina of embryonic and neonatal rats
- Source :
- Brain Research. 1029:24-33
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Fluoro-Jade (FJ) and Fluoro-Jade B (FJB) are fluorescein derivatives currently used to stain brain cells under degeneration. In this study, we investigated the FJ staining of nondegenerating cells in embryonic and neonatal rat brain and retina. In embryonic rat brain (embryonic day 15; E15), very intense staining of cells was observed. The number of FJ-stained cells and the intensity of staining decreased with increasing in animal age, being almost absent by postnatal day 16 (P16). Only a few cells in neonatal rat brain were in the process of cell death, as verified by the TUNEL technique. The FJ-stained cells in neonatal brain were positive for the neuronal marker neuronal nuclei antigen (NeuN). In retina, FJ stained mainly cells from the ganglion cell layer at P2 and the neuroblastic layer at P2 and P6. In contrast to FJ, FJB did not stain nondegenerating cells in embryonic and neonatal rats. These results show that in addition to staining degenerating brain cells, FJ also stains nondegenerating central nervous system cells in embryonic and neonatal stages.
- Subjects :
- Programmed cell death
Pathology
medicine.medical_specialty
Cell Survival
Organogenesis
Central nervous system
Retina
Pregnancy
In Situ Nick-End Labeling
medicine
Animals
Organic Chemicals
Rats, Wistar
Molecular Biology
Ganglion cell layer
Fluorescent Dyes
Brain Chemistry
Staining and Labeling
biology
General Neuroscience
Brain
Fluoresceins
Embryonic stem cell
Rats
Staining
medicine.anatomical_structure
Nerve Degeneration
biology.protein
Female
Neurology (clinical)
Neuron
NeuN
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1029
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....27ecd619927adb5c02f6a45d1f9ed446