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Molecular evaluation of vitamin D responsiveness of healthy young adults
- Source :
- The Journal of Steroid Biochemistry and Molecular Biology. 174:314-321
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Vitamin D3 has via its metabolites 25-hydroxyvitamin D3 (25(OH)D3) and 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) direct effects on the transcriptome and the epigenome of most human cells. In the VitDbol study we exposed 35 healthy young adults to an oral vitamin D3 dose (2000μg) or placebo and took blood samples directly before the supplementation as well as at days 1, 2 and 30. Within 24h the vitamin D3 intake raised the average serum levels of both 25(OH)D3 and 1,25(OH)2D3 by approximately 20%. However, we observed large inter-individual differences in these serum levels, reflected by the average ratios between 25(OH)D3 and 1,25(OH)2D3 concentrations ranging from 277 to 1365. Interestingly, average serum parathyroid hormone (PTH) levels increased at day 1 by some 10% but then decreased within the following four weeks to levels 5% below baseline. In peripheral blood mononuclear cells (PBMCs) that were isolated at the same time points we determined vitamin D-modulated chromatin accessibility by FAIRE-qPCR at selected genomic loci. This method is well suited to evaluate both short-term and long-term in vivo effects of vitamin D on the epigenome of human subjects. The differential vitamin D responsiveness of the VitDbol study participants was determined via individual changes in their PTH levels or chromatin accessibility in relation to alterations in 25(OH)D3 concentrations. This led to the segregation of the subjects into 14 high, 11 mid and 10 low responders. In summary, the vitamin D responsiveness classification provides additional information compared to a vitamin D status assessment based on single 25(OH)D3 serum measurements. The study was registered at Clinicaltrials.gov (NCT02063334).
- Subjects :
- Male
0301 basic medicine
Vitamin
medicine.medical_specialty
Calcitriol
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Parathyroid hormone
Biology
Biochemistry
Peripheral blood mononuclear cell
Calcitriol receptor
Young Adult
03 medical and health sciences
chemistry.chemical_compound
Endocrinology
Internal medicine
Vitamin D and neurology
medicine
Humans
Molecular Biology
Calcifediol
Cholecalciferol
Intracellular Signaling Peptides and Proteins
Vitamins
Cell Biology
3. Good health
rap GTP-Binding Proteins
030104 developmental biology
chemistry
Parathyroid Hormone
Leukocytes, Mononuclear
Molecular Medicine
Female
medicine.drug
Subjects
Details
- ISSN :
- 09600760
- Volume :
- 174
- Database :
- OpenAIRE
- Journal :
- The Journal of Steroid Biochemistry and Molecular Biology
- Accession number :
- edsair.doi.dedup.....27eb5e989f11babfd466fb1292415c8e