Back to Search
Start Over
A glucose oxidase immunoenzyme stain for the detection of viral antigen or antibody on nitrocellulose transfer blots
- Source :
- Journal of immunological methods. 72(1)
- Publication Year :
- 1984
-
Abstract
- Separation of mixtures of proteins by polyacrylamide gel electrophoresis followed by transfer of the proteins to support media such as nitrocellulose and detection by immunologic procedures provides a powerful analytic tool for assaying the antibody specificity of antisera or for following the purification of antigens. This technique requires fewer assumptions about antigenic solubility or antibody reactivity than immunoprecipitation methods. We present a glucose oxidase immunoenzyme staining procedure for protein blots and illustrate its use for detecting antibody to several viruses. The glucose oxidase immunoenzyme stain has a lower background than some peroxidase stains. We have detected as little as 1 μg/ml of antiviral antibody using this stain.
- Subjects :
- Paper
Immunology
Antibodies, Viral
Stain
Immunoenzyme Techniques
Glucose Oxidase
Antigen
Immunology and Allergy
Animals
Humans
Simplexvirus
Polyacrylamide gel electrophoresis
Antigens, Viral
Gel electrophoresis
Antiserum
biology
Staining and Labeling
Chemistry
Ferrets
Aleutian Mink Disease Virus
Collodion
Antiviral antibody
Molecular biology
Staining
Parainfluenza Virus 3, Human
Biochemistry
Mink
biology.protein
Electrophoresis, Polyacrylamide Gel
Antibody
Subjects
Details
- ISSN :
- 00221759
- Volume :
- 72
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of immunological methods
- Accession number :
- edsair.doi.dedup.....27e26a3c202f09898a59469ca089e941