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CCR6 Expression on B Cells Is Not Required for Clinical or Pathological Presentation of MOG Protein–Induced Experimental Autoimmune Encephalomyelitis despite an Altered Germinal Center Response
- Source :
- The Journal of Immunology. 207:1513-1521
- Publication Year :
- 2021
- Publisher :
- The American Association of Immunologists, 2021.
-
Abstract
- B cells have been implicated in the pathogenesis of multiple sclerosis, but the mechanisms that guide B cell activation in the periphery and subsequent migration to the CNS remain incompletely understood. We previously showed that systemic inflammation induces an accumulation of B cells in the spleen in a CCR6/CCL20-dependent manner. In this study, we evaluated the role of CCR6/CCL20 in the context of myelin oligodendrocyte glycoprotein (MOG) protein–induced (B cell–dependent) experimental autoimmune encephalomyelitis (EAE). We found that CCR6 is upregulated on murine B cells that migrate into the CNS during neuroinflammation. In addition, human B cells that migrate across CNS endothelium in vitro were found to be CCR6+, and we detected CCL20 production by activated CNS-derived human endothelial cells as well as a systemic increase in CCL20 protein during EAE. Although mice that lack CCR6 expression specifically on B cells exhibited an altered germinal center reaction in response to MOG protein immunization, CCR6-deficient B cells did not exhibit any competitive disadvantage in their migration to the CNS during EAE, and the clinical and pathological presentation of EAE induced by MOG protein was unaffected. These data, to our knowledge, provide new information on the role of B cell–intrinsic CCR6 expression in a B cell–dependent model of neuroinflammation.
- Subjects :
- Receptors, CCR6
Encephalomyelitis, Autoimmune, Experimental
Immunology
Blood Donors
chemical and pharmacologic phenomena
Spleen
C-C chemokine receptor type 6
Systemic inflammation
Myelin oligodendrocyte glycoprotein
Mice
Cell Movement
medicine
Animals
Humans
Immunology and Allergy
Cells, Cultured
Neuroinflammation
Mice, Knockout
B-Lymphocytes
Chemokine CCL20
biology
Experimental autoimmune encephalomyelitis
Endothelial Cells
Germinal center
hemic and immune systems
Germinal Center
medicine.disease
Recombinant Proteins
Mice, Inbred C57BL
CCL20
medicine.anatomical_structure
Blood-Brain Barrier
biology.protein
Female
Immunization
Myelin-Oligodendrocyte Glycoprotein
medicine.symptom
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 207
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....27deb72bf1d43d11a86ffde831c30220
- Full Text :
- https://doi.org/10.4049/jimmunol.2001413