Back to Search
Start Over
Substitution of the myristoylation signal of human immunodeficiency virus type 1 Pr55Gag with the phospholipase C-δ1 pleckstrin homology domain results in infectious pseudovirion production
- Source :
- The Journal of General Virology
- Publication Year :
- 2008
- Publisher :
- Microbiology Society, 2008.
-
Abstract
- The matrix domain (MA) of human immunodeficiency virus type 1 Pr55Gagis covalently modified with a myristoyl group that mediates efficient viral production. However, the role of myristoylation, particularly in the viral entry process, remains uninvestigated. This study replaced the myristoylation signal of MA with a well-studied phosphatidylinositol 4,5-biphosphate-binding plasma membrane (PM) targeting motif, the phospholipase C-δ1 pleckstrin homology (PH) domain. PH–Gag–Pol PM targeting and viral production efficiencies were improved compared with Gag–Pol, consistent with the estimated increases in Gag–PM affinity. Both virions were recovered in similar sucrose density-gradient fractions and had similar mature virion morphologies. Importantly, PH–Gag–Pol and Gag–Pol pseudovirions had almost identical infectivity, suggesting a dispensable role for myristoylation in the virus life cycle. PH–Gag–Pol might be useful in separating the myristoylation-dependent processes from the myristoylation-independent processes. This the first report demonstrating infectious pseudovirion production without myristoylated Pr55Gag.
- Subjects :
- viruses
Gene Products, gag
Biology
Phospholipase
Myristic Acid
Virus
Cell Line
Pseudovirion
Viral life cycle
Viral entry
Virology
Humans
Amino Acid Sequence
Protein Precursors
Myristoylation
Phospholipase C
Animal
Virion
Blood Proteins
Phosphoproteins
Fusion Proteins, gag-pol
Pleckstrin homology domain
Amino Acid Substitution
Biochemistry
HIV-1
lipids (amino acids, peptides, and proteins)
Phospholipase C delta
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....27dbf7a8424cc3fca84d383dbe97cabe