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Nucleolar organizer regions of megakaryocytes in chronic myeloproliferative disorders
- Source :
- Virchows Archiv A Pathological Anatomy and Histopathology. 420:441-445
- Publication Year :
- 1992
- Publisher :
- Springer Science and Business Media LLC, 1992.
-
Abstract
- To study megakaryocyte activation, the argyrophilic staining method of nucleolar organizer regions (AgNOR) has been applied to decalcified bone marrow biopsies of 16 individuals with no haematopoietic disorders and 59 patients with chronic myeloproliferative disease. Of the 59 patients, 18 had chronic myeloid leukaemia (CML), 21 chronic megakaryocytic granulocytic myelosis (CMGM), 13 polycythaemia vera (PV) and 7 essential thrombocythaemia (ET). The AgNOR number of megakaryocytes in CML was significantly lower, and in CMGM, PV and ET significantly higher than in healthy individuals. The high number and the clusters of fine-grained AgNORs of megakaryocytes in CMGM, PV and ET are suggestive of active, proliferating cells. The AgNOR number of megakaryocytes and the platelet counts of the patients did not show a convincing correlation. In CMGM, PV and ET the pyknotic, heterochromatinized megakaryocytes with narrow rims of cytoplasm called bare (nude) nuclei, possessed few, large AgNOR granules. The AgNOR staining of bare nuclei and the roughly identical number of granules found in CMGM, PV and ET indicate a common, active mechanism of apoptosis.
- Subjects :
- Polycythaemia
Pathology
medicine.medical_specialty
Biopsy
Biology
Pathology and Forensic Medicine
Megakaryocyte
Bone Marrow
hemic and lymphatic diseases
Nucleolus Organizer Region
medicine
Humans
Platelet
Molecular Biology
Myeloproliferative Disorders
Staining and Labeling
medicine.diagnostic_test
Cell Biology
General Medicine
medicine.disease
Nucleolar Organizer Region
Staining
Haematopoiesis
medicine.anatomical_structure
Chronic Disease
Nucleolus organizer region
Megakaryocytes
Subjects
Details
- ISSN :
- 14322307 and 01747398
- Volume :
- 420
- Database :
- OpenAIRE
- Journal :
- Virchows Archiv A Pathological Anatomy and Histopathology
- Accession number :
- edsair.doi.dedup.....27d2774e3ea8aea3ba67cd354d08c6b0