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Interleukin-18 enhances the production of interferon-gamma (IFN-γ) by allergen-specific and unspecific stimulated cord blood mononuclear cells
- Source :
- Cytokine. 25:172-178
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Background IL-18 is a pleiotropic cytokine involved in the polarisation of T-cell response. This study was performed to determine whether or not IL-18 is detectable in phytohemagglutinin (PHA) or betalactoglobulin (BLG) stimulated supernatants of cord blood mononuclear cells (CBMC) and to study the in vitro effect of IL-18 on the interferon (IFN)-γ and IL-13 release of CBMC of healthy neonates. Methods CBMC of neonates were isolated by Ficoll density centrifugation. The cytokines IFN-γ, IL-13 and IL-18 in the cell culture supernatants were measured using the ELISA technique following stimulation with a unspecific (PHA 20 μg/ml) and an allergen-specific stimulus (BLG 25 μg/ml). In order to study the in vitro effect of IL-18, CBMC were stimulated either with medium alone or with IL-18, IL-18 + PHA and IL-18 + BLG. Results IL-18 levels in supernatants of CBMC were low and did not vary significantly between unstimulated and PHA or BLG stimulated cell cultures (median 21.4; 23.5 and 15.5 pg/ml, respectively). IFN-γ and IL-13 levels were significantly higher in response to PHA and BLG (PHA: IFN-γ, 6154; IL-13, 4357; BLG: IFN-γ, 801; IL-13, 249 pg/ml) compared to unstimulated cell cultures. The addition of IL-18 to PHA or BLG stimulated CBMC significantly enhanced the IFN-γ release (PHA: 6154; PHA + IL-18: 13474, p =0.0001; BLG: 801; BLG + IL-18: 1077, p =0.008). In comparison to incubation without IL-18, the release of IL-13 was invariable or even reduced, when CBMC were stimulated with PHA + IL-18 (4026, p =0.16) or BLG + IL-18 (124, p =0.0001) compared to stimulation of CBMC with PHA (4357 pg/ml) or BLG (249 pg/ml) alone. Conclusions IL-18 is detectable in supernatants of CBMC. We observed a significant effect of IL-18 + PHA as well as IL-18 + BLG on IFN-γ release in vitro. Based on our findings we conclude that IL-18 could act as a strong TH1-inducing factor on stimulated CBMC also in vivo.
- Subjects :
- medicine.medical_treatment
Immunology
Ficoll
Stimulation
Lactoglobulins
Biology
Biochemistry
Peripheral blood mononuclear cell
Substrate Specificity
Interferon-gamma
In vivo
medicine
Humans
Immunology and Allergy
Interferon gamma
Phytohemagglutinins
Molecular Biology
Interleukin-13
Interleukin-18
Hematology
Allergens
Fetal Blood
Molecular biology
In vitro
Cytokine
Cell culture
Leukocytes, Mononuclear
medicine.drug
Subjects
Details
- ISSN :
- 10434666
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Cytokine
- Accession number :
- edsair.doi.dedup.....27bf9d1f9e386bdec85205aa1dd9a2e5