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Procoagulant properties of human MV3 melanoma cells
- Source :
- Brazilian Journal of Medical and Biological Research, Volume: 41, Issue: 2, Pages: 105-99, Published: 11 JAN 2008, Brazilian Journal of Medical and Biological Research v.41 n.2 2008, Brazilian Journal of Medical and Biological Research, Associação Brasileira de Divulgação Científica (ABDC), instacron:ABDC, Brazilian Journal of Medical and Biological Research, Vol 41, Iss 2, Pp 99-105 (2008)
- Publication Year :
- 2008
- Publisher :
- FapUNIFESP (SciELO), 2008.
-
Abstract
- A correlation between cancer and prothrombotic states has long been described. More recently, a number of studies have focused on the procoagulant mechanisms exhibited by tumor cells. In the present study, we dissected the molecular mechanisms responsible for the procoagulant activity of MV3, a highly aggressive human melanoma cell line. It was observed that tumor cells strongly accelerate plasma coagulation as a result of: i) expression of the blood clotting initiator protein, a tissue factor, as shown by flow cytometry and functional assays (factor Xa formation in the presence of cells and factor VIIa), and ii) direct activation of prothrombin to thrombin by cells, as evidenced by hydrolysis of the synthetic substrate, S-2238, and the natural substrate, fibrinogen. This ability was highly potentiated by the addition of exogenous factor Va, which functions as a co-factor for the enzyme factor Xa. In contrast, prothrombin activation was not observed when cells were previously incubated with DEGR-factor Xa, an inactive derivative of the enzyme. Moreover, a monoclonal antibody against bovine factor Xa reduced the prothrombin-converting activity of tumor cells. In conclusion, the data strongly suggest that MV3 cells recruit factor Xa from the culture medium, triggering an uncommon procoagulant mechanism.
- Subjects :
- Physiology
medicine.drug_class
Immunology
Biophysics
Ocean Engineering
Factor VIIa
Fibrinogen
Monoclonal antibody
Biochemistry
Thromboplastin
Flow cytometry
Tissue factor
Thrombin
Cell Line, Tumor
medicine
Humans
General Pharmacology, Toxicology and Pharmaceutics
lcsh:QH301-705.5
Melanoma
lcsh:R5-920
medicine.diagnostic_test
Chemistry
General Neuroscience
Blood clotting
Factor V
Cell Biology
General Medicine
Flow Cytometry
medicine.disease
Molecular biology
Neoplasm Proteins
Cysteine Endopeptidases
MV3 melanoma
lcsh:Biology (General)
Coagulation
Cell culture
Factor Xa
Prothrombin
lcsh:Medicine (General)
medicine.drug
Subjects
Details
- ISSN :
- 1414431X and 0100879X
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Brazilian Journal of Medical and Biological Research
- Accession number :
- edsair.doi.dedup.....27b2f03dc3c9722ce49e43e62cfd12bc