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An archipelago of CpG islands in Xq28: identification and fine mapping of 20 new CpG islands of the human X chromosome

Authors :
Michele D'Urso
Filippo Tamanini
Giuseppe Palmieri
Elisabeth Gimbo
Annemarie Poustka
Elena Maestrini
Petra Kioschis
Paola Marinelli
Daniela Toniolo
Carla Tribioli
Source :
ResearcherID
Publication Year :
1992
Publisher :
Oxford University Press (OUP), 1992.

Abstract

19 probes for CpG islands, mapping to Xq28, have been used as probes to construct a physical map of genes of this band of the human X chromosome. A total of 22 CpG islands have been precisely mapped in respect to known loci along the 9-10 Mb of Xq28. The fine mapping of such a large number of CpG islands has demonstrated that also in gene rich Giemsa light bands, like Xq28, gene distribution is non uniform: the CpG islands are clustered in the distal portion of the band in a 2 Mb region between the G6PD gene and the DXS15 locus. Moreover, 16 CpG islands were found between the G6PD and the RCP/GCP genes, a region of DNA of only about 300 kb. If this structural organization has a biological function it has yet to be determined. However, the isolation of large genomic regions enriched in gene sequences and the availability of cosmid or YAC contigs will provide the means to test the significance of such gene organization, as well as the material for large sequencing projects and gene search, for the identification of candidate genes for inherited disorders mapped to Xq28 and for comparative mapping.

Details

ISSN :
14602083 and 09646906
Volume :
1
Database :
OpenAIRE
Journal :
Human Molecular Genetics
Accession number :
edsair.doi.dedup.....279db8cfa33defedc4a2a350a7d06fa8
Full Text :
https://doi.org/10.1093/hmg/1.4.275