Back to Search
Start Over
Usefulness of myeloperoxidase as a biomarker for the ranking of pulmonary toxicity of nanomaterials
- Source :
- Particle and Fibre Toxicology, Vol 15, Iss 1, Pp 1-14 (2018), Particle and Fibre Toxicology
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Background In order to examine whether myeloperoxidase (MPO) can be a useful marker for evaluating the pulmonary toxicity of nanomaterials, we analyzed MPO protein in bronchoalveolar lavage fluid (BALF) samples obtained from previous examinations of a rat model. In those examinations we performed intratracheal instillation exposures (dose: 0.2–1.0 mg) and inhalation exposures (exposure concentration: 0.32–10.4 mg/m3) using 9 and 4 nanomaterials with different toxicities, respectively. Based on those previous studies, we set Nickel oxide nanoparticles (NiO), cerium dioxide nanoparticles (CeO2), multi wall carbon nanotubes with short or long length (MWCNT (S) and MWCNT (L)), and single wall carbon nanotube (SWCNT) as chemicals with high toxicity; and titanium dioxide nanoparticles (TiO2 (P90) and TiO2 (Rutile)), zinc oxide nanoparticles (ZnO), and toner with external additives including nanoparticles as chemicals with low toxicity. We measured the concentration of MPO in BALF samples from rats from 3 days to 6 months following a single intratracheal instillation, and from 3 days to 3 months after the end of inhalation exposure. Results Intratracheal instillation of high toxicity NiO, CeO2, MWCNT (S), MWCNT (L), and SWCNT persistently increased the concentration of MPO, and inhalation of NiO and CeO2 increased the MPO in BALF. By contrast, intratracheal instillation of low toxicity TiO2 (P90), TiO2 (Rutile), ZnO, and toner increased the concentration of MPO in BALF only transiently, and inhalation of TiO2 (Rutile) and ZnO induced almost no increase of the MPO. The concentration of MPO correlated with the number of total cells and neutrophils, the concentration of chemokines for neutrophils (cytokine-induced neutrophil chemoattractant (CINC)-1 and heme oxygenase (HO)-1), and the activity of released lactate dehydrogenase (LDH) in BALF. The results from the receiver operating characteristics (ROC) for the toxicity of chemicals by the concentration of MPO proteins in the intratracheal instillation and inhalation exposures showed that the largest areas under the curves (AUC) s in both examinations occurred at 1 month after exposure. Conclusion These data suggest that MPO can be a useful biomarker for the ranking of the pulmonary toxicity of nanomaterials, especially at 1 month after exposure, in both intratracheal instillation and inhalation exposure. Electronic supplementary material The online version of this article (10.1186/s12989-018-0277-x) contains supplementary material, which is available to authorized users.
- Subjects :
- Male
0301 basic medicine
Neutrophils
Pulmonary toxicity
Health, Toxicology and Mutagenesis
lcsh:Industrial hygiene. Industrial welfare
010501 environmental sciences
Pharmacology
Toxicology
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
lcsh:RA1190-1270
Lactate dehydrogenase
Macrophages, Alveolar
medicine
Animals
Lung
Peroxidase
lcsh:Toxicology. Poisons
0105 earth and related environmental sciences
Inhalation exposure
Inhalation Exposure
Myeloperoxidase
Inhalation
medicine.diagnostic_test
biology
Research
Biomarker
General Medicine
Nanomaterial
Rats, Inbred F344
Heme oxygenase
030104 developmental biology
Bronchoalveolar lavage
chemistry
Toxicity
biology.protein
Rat
Nanoparticles
Chemokines
Bronchoalveolar Lavage Fluid
Intratracheal instillation
Biomarkers
lcsh:HD7260-7780.8
Subjects
Details
- ISSN :
- 17438977
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Particle and Fibre Toxicology
- Accession number :
- edsair.doi.dedup.....2773e1b8ab141a773c82218f252fb3d2