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Different processing of chromogranin B into GAWK-immunoreactive fragments in the bovine adrenal medulla and pituitary gland
- Source :
- Life sciences. 43(23)
- Publication Year :
- 1988
-
Abstract
- Summary Chromogranin B 420–493(GAWK)-like immunoreactivity (chromogranin B (420–493)-LI) was determined by radio-immunoassay using two different rabbit antisera, one raised against chromogranin B (420–436) (GAWK 1–17) (Ab420–436) and the other against chromogranin B 439–457 (GAWK 20–38) (Ab439–457), in bovine and human tissues. Chromogranin B (420–493)-LI was present in the bovine adrenal medulla chromaffin granules as well as in the anterior pituitary gland and was released from the cultured bovine chromaffin cells by stimulation with high K + or nicotine. Chromogranin B (420–493)-LI present in the bovine tissues was detected using Ab420–436 but was not detected using Ab439–457. In the human tissues, chromogranin B (420–493)-LI was detected using Ab420–436 as well as Ab439–457. This suggests that the amino acid sequence of this region (chromogranin B 439–457) is different between human and bovine. On the gel permeation chromatography, chromogranin B (420–493)-LI was eluted at the void volume in the bovine adrenal medulla and at an apparent molecular weight of 4000 in the anterior pituitary gland. On the reverse-phase high-performance liquid chromatography, multiple peaks of chromogranin B (420–493)-LI was detected in the bovine adrenal medulla while one component of chromogranin B (420–493)-LI was found in the anterior pituitary gland. These results suggest that chromogranin B is processed into small fragments of chromogranin B (420–493)-LIs and that this processing is tissue-specific.
- Subjects :
- endocrine system
Pituitary gland
medicine.medical_specialty
Nicotine
endocrine system diseases
Stimulation
Nerve Tissue Proteins
Biology
General Biochemistry, Genetics and Molecular Biology
Anterior pituitary
Pituitary Gland, Anterior
Internal medicine
medicine
Chromogranins
Animals
Humans
Chromaffin Granules
Tissue Distribution
General Pharmacology, Toxicology and Pharmaceutics
neoplasms
Peptide sequence
Medulla
Cells, Cultured
Chromatography, High Pressure Liquid
Antiserum
Immunoassay
Chromogranin A
General Medicine
digestive system diseases
Peptide Fragments
Molecular Weight
medicine.anatomical_structure
Endocrinology
Adrenal Medulla
biology.protein
Chromatography, Gel
Potassium
Cattle
Adrenal medulla
Chromogranin B
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 43
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Life sciences
- Accession number :
- edsair.doi.dedup.....276f2f611097a0b2326a9cd39c83a114