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Near-infrared double-illumination optical-resolution photoacoustic microscopy
- Source :
- Journal of biophotonicsREFERENCES. 14(3)
- Publication Year :
- 2020
-
Abstract
- Label-free chemical bond imaging is of great importance in biology and medicine. Photoacoustic imaging at the third near-infrared windows (1600-1870 nm, nearinfrared-III) provides a stable molecular vibrational imaging tool for lipid-rich tissue owing to the first overtone transition of the C-H bond at 1.7 _m. However, lacking high-energy pulsed laser sources at 1.7 _m and the strong water absorption significantly limit the signal-to-noise ratio of the lipid imaging, especially for thin lipid tissues. To circumvent this barrier, we develop near-infrared-III double-illumination optical-resolution photoacoustic microscopy (DIOR-PAM) for improving the sensitivity of label-free lipid imaging. Using the same laser, DIOR-PAM can enhance the sensitivity by nearly 100%, which we prove in the Monte Carlo simulation.We experimentally demonstrated 50%i100% sensitivity enhancement on non-biological and biological lipid-rich samples. This article is protected by copyright. All rights reserved.
- Subjects :
- Materials science
Infrared Rays
Overtone
Monte Carlo method
General Physics and Astronomy
Signal-To-Noise Ratio
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
law.invention
010309 optics
Photoacoustic Techniques
Photoacoustic microscopy
law
0103 physical sciences
General Materials Science
Sensitivity (control systems)
Lighting
Microscopy
business.industry
Spectrum Analysis
010401 analytical chemistry
Near-infrared spectroscopy
Resolution (electron density)
General Engineering
General Chemistry
Laser
0104 chemical sciences
Chemical bond
Optoelectronics
business
Subjects
Details
- ISSN :
- 18640648
- Volume :
- 14
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of biophotonicsREFERENCES
- Accession number :
- edsair.doi.dedup.....276aff3427254ce43896a10d5a5ba119