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Artificial Skin - Culturing of Different Skin Cell Lines for Generating an Artificial Skin Substitute on Cross-Weaved Spider Silk Fibres
- Source :
- PLoS ONE 6 (2011), Nr. 7, PLoS ONE, Vol 6, Iss 7, p e21833 (2011), PLoS ONE
- Publication Year :
- 2011
- Publisher :
- San Francisco : Public Library Science, 2011.
-
Abstract
- Background: In the field of Plastic Reconstructive Surgery the development of new innovative matrices for skin repair is in urgent need. The ideal biomaterial should promote attachment, proliferation and growth of cells. Additionally, it should degrade in an appropriate time period without releasing harmful substances, but not exert a pathological immune response. Spider dragline silk from Nephila spp meets these demands to a large extent. Methodology/Principal Findings: Native spider dragline silk, harvested directly out of Nephila spp spiders, was woven on steel frames. Constructs were sterilized and seeded with fibroblasts. After two weeks of cultivating single fibroblasts, keratinocytes were added to generate a bilayered skin model, consisting of dermis and epidermis equivalents. For the next three weeks, constructs in co-culture were lifted on an originally designed setup for air/liquid interface cultivation. After the culturing period, constructs were embedded in paraffin with an especially developed program for spidersilk to avoid supercontraction. Paraffin cross-sections were stained in Haematoxylin & Eosin (H&E) for microscopic analyses. Conclusion/Significance: Native spider dragline silk woven on steel frames provides a suitable matrix for 3 dimensional skin cell culturing. Both fibroblasts and keratinocytes cell lines adhere to the spider silk fibres and proliferate. Guided by the spider silk fibres, they sprout into the meshes and reach confluence in at most one week. A well-balanced, bilayered cocultivation in two continuously separated strata can be achieved by serum reduction, changing the medium conditions and the cultivation period at the air/liquid interphase. Therefore spider silk appears to be a promising biomaterial for the enhancement of skin regeneration.
- Subjects :
- Keratinocytes
Anatomy and Physiology
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
lcsh:Medicine
Biocompatible Materials
bombyx-mori
in-vivo
nephila-clavipes araneae
Tissue engineering
ddc:590
Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie)
Spider silk
lcsh:Science
Cells, Cultured
Skin
degradation
Burn Management
Skin repair
Multidisciplinary
Tissue Scaffolds
biology
Chemistry
Spiders
SILK
medicine.anatomical_structure
scaffolds
Medicine
Research Article
Biotechnology
biomaterials
Histology
Biomedical Engineering
Silk
Bioengineering
Artificial skin
web
dragline silk
Dermis
Bombyx mori
ddc:570
medicine
Animals
Biology
Skin, Artificial
Spider
Tissue Engineering
lcsh:R
fungi
Fibroblasts
Reconstructive Surgery
biology.organism_classification
Coculture Techniques
escherichia-coli
Surgery
lcsh:Q
protein
Biomedical engineering
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE 6 (2011), Nr. 7, PLoS ONE, Vol 6, Iss 7, p e21833 (2011), PLoS ONE
- Accession number :
- edsair.doi.dedup.....276aaa23e0a91d1bd091a26ad02c2bb6
- Full Text :
- https://doi.org/10.15488/296