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Modulating Self-Assembly of DNA Crystals with Rationally Designed Agents
- Source :
- Angewandte Chemie. 130:16767-16770
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- This manuscript reports a strategy for controlling the crystallization kinetics and improving the quality of engineered self-assembled 3D DNA crystals. Growing large, high-quality biomacromolecule crystals is critically important for determining the 3D structures of biomacromolecules. It often presents a great challenge to structural biologists. Herein, we introduce a rationally designed agent to modulate the crystallization process. Under such conditions, fewer, but larger, crystals that yield diffraction patterns of modestly higher resolution are produced compared with the crystals from conditions without the modulating agent. We attribute the improvement to a smaller number of nuclei and slow growth rate of crystallization. This strategy is expected to be generally applicable for crystallization of other biomacromolecules.
- Subjects :
- Models, Molecular
Materials science
Nanotechnology
02 engineering and technology
010402 general chemistry
01 natural sciences
Catalysis
law.invention
Crystallization kinetics
chemistry.chemical_compound
Dna nanostructures
law
DNA nanotechnology
Crystallization
Base Sequence
010405 organic chemistry
General Chemistry
DNA
General Medicine
021001 nanoscience & nanotechnology
0104 chemical sciences
chemistry
Nanoparticles
Nucleic Acid Conformation
Slow Growth Rate
Self-assembly
0210 nano-technology
Subjects
Details
- ISSN :
- 00448249
- Volume :
- 130
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie
- Accession number :
- edsair.doi.dedup.....276950ff1f514d0bf8bb38cbd50fdc17