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Tailoring of silica-based nanoporous pod by spermidine multi-activity
- Source :
- Scientific Reports
- Publication Year :
- 2020
-
Abstract
- Ubiquitous in nature, polyamines (PAs) are a class of low-molecular aliphatic amines critically involved in cell growth, survival and differentiation. The polycation behavior is validated as a successful strategy in delivery systems to enhance oligonucleotide loading and cellular uptake. In this study, the chemical features and the functional roles of the PA spermidine are synergistically exploited in the synthesis and bioactive functionalization of SiO2-based structures. Inspired by biosilicification, the role of spermidine is assessed both as catalyst and template in a biomimetic one-pot synthesis of dense silica-based particles (SPs) and as a competitive agent in an interfacial reassembly strategy, to empty out SPs and generate spermidine-decorated hollow silica nanoporous pods (spd-SNPs). Spermidine bioactivity is then employed for targeting tumor cell over-expressed polyamine transport system (PTS) and for effective delivery of functional miRNA into melanoma cells. Spermidine decoration promotes spd-SNP cell internalization mediated by PTS and along with hollow structure enhances oligonucleotide loading. Accordingly, the functional delivery of the tumor suppressor miR-34a 3p resulted in intracellular accumulation of histone-complexed DNA fragments associated with apoptosis. Overall, the results highlight the potential of spd-SNP as a multi-agent anticancer therapy.
- Subjects :
- 0301 basic medicine
polyamines
media_common.quotation_subject
Cell
02 engineering and technology
Article
03 medical and health sciences
chemistry.chemical_compound
medicine
microRNA. drug delivery
Internalization
media_common
Multidisciplinary
sylica, nanoparticles, polyamines, microRNA. drug delivery
Polyamine transport
Oligonucleotide
Nanoporous
021001 nanoscience & nanotechnology
Spermidine
030104 developmental biology
medicine.anatomical_structure
chemistry
Drug delivery
Biophysics
Surface modification
nanoparticles
sylica
Biomaterials - cells
0210 nano-technology
Biomedical materials
Intracellular
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....276043ec8ad8f7ef442ace1b8de194d2