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Human sorCS1 binds sortilin and hampers its cellular functions

Authors :
Guido Hermey
Peder Madsen
Jørgen Gliemann
Esben S. Sørensen
Erik Ilsø Christensen
Thaneas Prabakaran
Claus Munck Petersen
Jakob Vejby Larsen
Source :
Larsen, J V, Hermey, G, Sørensen, E S, Prabakaran, T, Christensen, E I, Gliemann, J, Madsen, P & Petersen, C M 2014, ' Human sorCS1 binds sortilin and hampers its cellular functions ', Biochemical Journal, vol. 457, no. 2, pp. 277–288 . https://doi.org/10.1042/BJ20130386
Publication Year :
2013
Publisher :
Portland Press Ltd., 2013.

Abstract

Sortilin and sorCS1 [sortilin-related Vps10p (vacuolar protein sorting/targeting protein 10) domain-containing receptor 1], both members of the Vps10p-D (Vps10p-domain) receptor family, are synthesized as precursor proteins and are converted into their mature form by enzymatic cleavage of a short N-terminal propeptide. SorCS1 does not bind its propeptide, but sortilin is able to bind not just its own propeptide, but also that of sorCS1. In the present study we show that the propeptide region of sorCS1 contains two separate sites for binding to sortilin and that only one of these sites is removed from human (as opposed to mouse) sorCS1 during processing. This leaves mature human sorCS1 with a sortilin-binding N-terminus, which allows formation of a complex between the two receptors in solution and on cell membranes. Furthermore, we find that the interaction with sorCS1 has a pronounced effect on sortilin's ability to mediate the cellular uptake of alternative ligands, and to hamper its facilitation of CNTF (ciliary neutrophic factor) signalling and the induction of phosphorylated STAT3 (signal transducer and activator of transcription 3). Thus the present study reveals a novel regulatory mechanism and suggest an entirely new role for sorCS1 as a modulator of sortilin function.

Details

ISSN :
14708728 and 02646021
Volume :
457
Database :
OpenAIRE
Journal :
Biochemical Journal
Accession number :
edsair.doi.dedup.....2759d91b0bde3aff3e742fb9ff9bb491