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Tolerogenic properties of CD206+ macrophages appeared in the sublingual mucosa after repeated antigen-painting
- Source :
- International immunology. 32(8)
- Publication Year :
- 2019
-
Abstract
- The sublingual mucosa (SLM) in the oral cavity is utilized as the site for sublingual immunotherapy to induce tolerance against allergens. We previously reported that CD206+ round-type macrophage-like cells were induced in the SLM after repeated antigen (e.g. cedar pollen or fluorescein isothiocyanate (FITC))-painting. In this study, we examined the phenotypic and functional properties of CD206+ cells induced by repeated FITC-painting on the SLM. CD206+ cells after the repeated FITC-painting possessed a macrophage-like CD11b+Ly6C+ F4/80+CD64+ phenotype and expressed TIM-4, which was expressed in tolerogenic tissue-resident macrophages, at a high level. SLM CD206+ cells preferentially expressed molecules related to endocytosis and homeostatic processes, including the novel B7 family of immune checkpoint molecules, as assessed by microarray analyses. SLM CD206+ cells showed preferential expression of M2-related genes such as Fizz1, Aldh1a1 and Aldh1a2 but not Ym-1 and Arginase-1. A CD206+ cell-rich status inhibited OVA-specific CD4+ T-cell responses but reciprocally enhanced the proportion of both IL-10+CD4+ cells and Foxp3+ regulatory T-cells in regional lymph nodes. Co-culture of CD206+ cells with dendritic cells (DCs) showed that IL-12 production was suppressed in DCs concurrent with the decline of the MHC class IIhiCD86+ population, which was restored by neutralization of IL-10. These results demonstrate SLM CD206+ cells show the feature of tolerogenic macrophages and down-regulate the antigen-presenting cell function of mature DCs resulting in the inhibition of CD4+ T-cell responses.
- Subjects :
- Cryptomeria
Immunology
Population
Receptors, Cell Surface
chemistry.chemical_compound
Mice
Antigen
MHC class I
Immunology and Allergy
Animals
Lectins, C-Type
Fluorescein isothiocyanate
education
Mouth Floor
education.field_of_study
Mice, Inbred BALB C
Mucous Membrane
integumentary system
biology
Macrophages
FOXP3
General Medicine
Allergens
Cell biology
Interleukin 10
Mannose-Binding Lectins
Integrin alpha M
chemistry
Macrophage-1 antigen
biology.protein
Pollen
Female
Fluorescein-5-isothiocyanate
Mannose Receptor
Subjects
Details
- ISSN :
- 14602377
- Volume :
- 32
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- International immunology
- Accession number :
- edsair.doi.dedup.....2745ba358b47f1a79d049861d144c50e