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Functionalized NIR‐II Semiconducting Polymer Nanoparticles for Single‐cell to Whole‐Organ Imaging of PSMA‐Positive Prostate Cancer
- Source :
- Small. 16:2001215
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Development of molecular probes holds great promise for early diagnosis of aggressive prostate cancer. Here, 2-[3-(1,3-dicarboxypropyl) ureido] pentanedioic acid (DUPA)-conjugated ligand and bis-isoindigo-based polymer (BTII) are synthesized to formulate semiconducting polymer nanoparticles (BTII-DUPA SPN) as a prostate-specific membrane antigen (PSMA)-targeted probe for prostate cancer imaging in the NIR-II window. Insights into the interaction of the imaging probes with the biological targets from single cell to whole organ are obtained by transient absorption (TA) microscopy and photoacoustic (PA) tomography. At single-cell level, TA microscopy reveals the targeting efficiency, kinetics, and specificity of BTII-DUPA SPN to PSMA-positive prostate cancer. At organ level, PA tomographic imaging of BTII-DUPA SPN in the NIR-II window demonstrates superior imaging depth and contrast. By intravenous administration, BTII-DUPA SPN demonstrates selective accumulation and retention in the PSMA-positive tumor, allowing noninvasive PA detection of PSMA overexpressing prostate tumors in vivo. The distribution of nanoparticles inside the tumor tissue is further analyzed through TA microscopy. These results collectively demonstrate BTII-DUPA SPN as a promising probe for prostate cancer diagnosis by PA tomography.
- Subjects :
- Diagnostic Imaging
Male
Polymers
Cell
02 engineering and technology
urologic and male genital diseases
010402 general chemistry
01 natural sciences
Biomaterials
Prostate cancer
In vivo
Cell Line, Tumor
Microscopy
medicine
Humans
Distribution (pharmacology)
General Materials Science
Chemistry
Prostatic Neoplasms
General Chemistry
021001 nanoscience & nanotechnology
Ligand (biochemistry)
medicine.disease
0104 chemical sciences
medicine.anatomical_structure
Cancer research
Nanoparticles
Tomography
0210 nano-technology
Molecular probe
Biotechnology
Subjects
Details
- ISSN :
- 16136829 and 16136810
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Small
- Accession number :
- edsair.doi.dedup.....271db26c7beb315917376823776739b0