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Changes in Single K+ Channel Behavior Induced by a Lipid Phase Transition
- Source :
- Biophysical journal, (2010)., info:cnr-pdr/source/autori:Seeger H.M., L. Aldrovandi, A. Alessandrini, and P. Facci/titolo:Changes in Single K+ Channel Behavior Induced by a Lipid Phase Transition/doi:/rivista:Biophysical journal (Print)/anno:2010/pagina_da:/pagina_a:/intervallo_pagine:/volume
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- We show that the activity of an ion channel is correlated with the phase state of the lipid bilayer hosting the channel. By measuring unitary conductance, dwell times, and open probability of the K+ channel KcsA as a function of temperature in lipid bilayers composed of POPE and POPG in different relative proportions, we obtain that all those properties show a trend inversion when the bilayer is in the transition region between the liquid-disordered and the solid-ordered phase. These data suggest that the physical properties of the lipid bilayer influence ion channel activity likely via a fine-tuning of its conformations. In a more general interpretative framework, we suggest that other parameters such as pH, ionic strength, and the action of amphiphilic drugs can affect the physical behavior of the lipid bilayer in a fashion similar to temperature changes resulting in functional changes of transmembrane proteins.
- Subjects :
- MELTING TRANSITION
Phase transition
POTASSIUM CHANNELS
TEMPERATURE-DEPENDENCE
MECHANICAL-PROPERTIES
MEMBRANE-PROTEINS
Potassium Channels
Time Factors
Lipid Bilayers
Analytical chemistry
KcsA potassium channel
Biophysics
Phase Transition
Bacterial Proteins
Lipid bilayer phase behavior
Lipid bilayer
Ion channel
Chemistry
Bilayer
Phosphatidylethanolamines
Membrane
Electric Conductivity
Temperature
Phosphatidylglycerols
Lipid bilayer mechanics
Chemical physics
Ionic strength
Streptomyces lividans
Ion Channel Gating
Subjects
Details
- ISSN :
- 00063495
- Volume :
- 99
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Biophysical Journal
- Accession number :
- edsair.doi.dedup.....271acdf941b5c6766d20a7690123f62c
- Full Text :
- https://doi.org/10.1016/j.bpj.2010.10.042