Formalin-fixed paraffin-embedded tissues for microbiome analysis in rainbow trout (Oncorhynchus mykiss)

Authors :: Roberto, Cruz-Flores
Mónica, Hernández Rodríguez
Jesús Salvador Olivier Guirado, Flores
Arun K, Dhar
Source :: Journal of Microbiological Methods. 192:106389
Publication Year :: 2022
Publisher :: Elsevier BV, 2022.
Abstract: The gut microbiomes of rainbow trout (Oncorhynchus mykiss) reared at 16° and 22 °C were determined using formalin-fixed paraffin-embedded tissues (FFPE) and compared to fresh frozen tissue. The data revealed microbiomes could be successfully determined using FFPE tissue opening a new horizon in studying intestinal microbiota using archived histological samples.

Subjects :: Cryopreservation
Microbiology (medical)
Paraffin Embedding
Tissue Fixation
Base Sequence
Temperature
High-Throughput Nucleotide Sequencing
Microbiology
Gastrointestinal Microbiome
Fixatives
Formaldehyde
Oncorhynchus mykiss
RNA, Ribosomal, 16S
Animals
Molecular Biology

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