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p53-mediated DNA renaturation can mimic strand exchange
- Source :
- Nucleic Acids Research. 25:4004-4012
- Publication Year :
- 1997
- Publisher :
- Oxford University Press (OUP), 1997.
-
Abstract
- The process of strand exchange is considered to be the hallmark of DNA recombination. Proteins known to carry out such exchange are believed to operate via one or the other of two mechanisms. RecA-like proteins promote the formation of a three-stranded or triplex synaptic intermediate in which strand exchange occurs, whereas other proteins would allow the coordinated exonucleolytic degradation of one strand in the duplex DNA and its replacement by an invading strand of similar sequence and polarity. In view of properties ascribed to it, we have attempted to determine whether p53 belongs to one or the other of these groups of proteins. The in vitro assay used relies on a double-stranded (ds) oligonucleotide (oligo 1+2) and a single-stranded (ss) oligonucleotide (oligo 3), part of which is complementary to oligo 1. The data collected suggest that, under the conditions of the assay, oligo 1+2 undergoes partial denaturation; p53 then catalyzes renaturation of oligo 1 with oligo 3, rather than true strand exchange. Since p53 is not known for being able to 'melt' DNA, it would seem unlikely that this protein would effect strand exchange in vivo without assistance from another, denaturing, protein.
- Subjects :
- Cations, Divalent
Oligonucleotides
DNA, Single-Stranded
Spodoptera
Biology
DNA-binding protein
law.invention
chemistry.chemical_compound
D-loop
law
Escherichia coli
Genetics
Animals
Magnesium
Denaturation (biochemistry)
Recombination, Genetic
Oligonucleotide
DNA
DNA-Binding Proteins
Rec A Recombinases
Biochemistry
chemistry
Coding strand
Nucleic Acid Renaturation
Recombinant DNA
Tumor Suppressor Protein p53
Baculoviridae
Research Article
Subjects
Details
- ISSN :
- 13624962
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....26f6611b32edf7c67df099e25bee0997