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HIV-1 molecular epidemiology and drug resistance-associated mutations among treatment-naïve blood donors in China
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Surveillance of human immunodeficiency virus (HIV) molecular diversity and drug resistance-associated mutations (DRMs) among treatment-naïve blood donors is critical for monitoring viral evolution and blood safety. From 2016-2017, 199 plasma samples were collected from 24 blood centers and confirmed as HIV viral load positive or serologically reactive in National Centers for Clinical Laboratories (NCCL), of which 179 were sequenced and subtyped in the gag, protease (PR)-reverse transcriptase (RT), integrase (IN) and/or envelope (env) regions. DRMs in PR-RT and IN regions were analyzed in Stanford HIVdb Program. The majority of subtypes were circulating recombinant form (CRF) 07_BC (34.6%) and CRF01_AE (32.4%); many unique recombinant forms (URFs) (39, 21.8%) and other rare CRFs were observed in the study. Notably, CRF02_AG and CRF06_cpx strains typically found in Africa were firstly identified amongst Chinese blood donors. DRMs were common, with 28 of 179 (15.6%) specimens carrying DRMs, including the PR N88S and RT K103N mutations, which have been implicated in elevated resistance to antiretroviral drugs. Furthermore, 4 HIV-1 isolates (2.4%, 4/168) had surveillance drug-resistance mutation (SDRM), including 3 nonnucleosidereverse transcriptase inhibitors (NNRTI) SDRMs (1 K101E, 2 K103N) and 1 protease inhibitor (PI) SDRM (M46I). The HIV viral diversity among blood donors observed in this study suggest that ongoing HIV-1 recombination is becoming progressively complex in China, and lots of DRMs found in the study exacerbate the primary drug resistance landscape, which highlight the necessity of timely genotypic drug resistance monitoring and molecular surveillance of HIV-1 among blood donors.
- Subjects :
- Adult
Male
0301 basic medicine
Viral epidemiology
Adolescent
Genotype
030106 microbiology
lcsh:Medicine
HIV Infections
Genome, Viral
Drug resistance
Article
Young Adult
03 medical and health sciences
Antiretroviral Therapy, Highly Active
Drug Resistance, Viral
Humans
Protease inhibitor (pharmacology)
Clinical microbiology
lcsh:Science
Phylogeny
Aged
Molecular Epidemiology
Retrovirus
Multidisciplinary
biology
Molecular epidemiology
lcsh:R
virus diseases
Middle Aged
Virology
Reverse transcriptase
Integrase
030104 developmental biology
Viral evolution
Mutation
HIV-1
biology.protein
Female
lcsh:Q
Viral load
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....26f3dabf1c165727259a0b81fc54f8b3