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Comparative proteomic analysis of Lactobacillus plantarum for the identification of key proteins in bile tolerance
- Source :
- BMC Microbiology (11), 63. (2011), BMC Microbiology, BMC Microbiology, BioMed Central, 2011, 11, pp.63. ⟨10.1186/1471-2180-11-63⟩, BMC Microbiology, 11:63. BMC, BMC Microbiology, Vol 11, Iss 1, p 63 (2011)
- Publication Year :
- 2011
-
Abstract
- Lactic acid bacteria are commonly marketed as probiotics based on their putative or proven health-promoting effects. These effects are known to be strain specific but the underlying molecular mechanisms remain poorly understood. Therefore, unravelling the determinants behind probiotic features is of particular interest since it would help select strains that stand the best chance of success in clinical trials. Bile tolerance is one of the most crucial properties as it determines the ability of bacteria to survive in the small intestine, and consequently their capacity to play their functional role as probiotics. In this context, the objective of this study was to investigate the natural protein diversity within the Lactobacillus plantarum species with relation to bile tolerance, using comparative proteomics.<br />Bile tolerance properties of nine L. plantarum strains were studied in vitro. Three of them presenting different bile tolerance levels were selected for comparative proteomic analysis: L. plantarum 299 V (resistant), L. plantarum LC 804 (intermediate) and L. plantarum LC 56 (sensitive). Qualitative and quantitative differences in proteomes were analyzed using two-dimensional electrophoresis (2-DE), tryptic digestion, liquid chromatography-mass spectrometry analysis and database search for protein identification. Among the proteins correlated with differences in the 2-DE patterns of the bacterial strains, 15 have previously been reported to be involved in bile tolerance processes. The effect of a bile exposure on these patterns was investigated, which led to the identification of six proteins that may be key in the bile salt response and adaptation in L. plantarum: two glutathione reductases involved in protection against oxidative injury caused by bile salts, a cyclopropane-fatty-acyl-phospholipid synthase implicated in maintenance of cell envelope integrity, a bile salt hydrolase, an ABC transporter and a F0F1-ATP synthase which participate in the active removal of bile-related stress factors.<br />These results showed that comparative proteomic analysis can help understand the differential bacterial properties of lactobacilli. In the field of probiotic studies, characteristic proteomic profiles can be identified for individual properties that may serve as bacterial biomarkers for the preliminary selection of strains with the best probiotic potential.
- Subjects :
- Proteome
lcsh:QR1-502
medicine.disease_cause
Proteomics
lcsh:Microbiology
Mass Spectrometry
law.invention
Probiotic
law
SALT HYDROLASE
Electrophoresis, Gel, Two-Dimensional
OXIDATIVE STRESS
HUMAN MICROBIOME
MESH: Stress, Physiological
LISTERIA-MONOCYTOGENES
MESH: Bacterial Proteins
SUBSP LACTIS
0303 health sciences
MESH: Gene Expression Regulation, Bacterial
Vegetal Biology
Human microbiome
lactobacillus plantarum
Bifidobacterium animalis
Anti-Bacterial Agents
MESH: Proteome
Biochemistry
ESCHERICHIA-COLI
MESH: Lactobacillus plantarum
Research Article
analyse du métabolisme
Microbiology (medical)
DNA-BINDING
bactérie lactique
Context (language use)
metabolism analysis
bactérie probiotique
MESH: Bile Acids and Salts
Biology
Microbiology
2-DIMENSIONAL ELECTROPHORESIS
Bile Acids and Salts
03 medical and health sciences
Bacterial Proteins
Stress, Physiological
MESH: Anti-Bacterial Agents
medicine
Escherichia coli
030304 developmental biology
MESH: Mass Spectrometry
BIFIDOBACTERIUM-ANIMALIS
030306 microbiology
Gene Expression Regulation, Bacterial
biology.organism_classification
MESH: Electrophoresis, Gel, Two-Dimensional
PROPIONIBACTERIUM-FREUDENREICHII
lactic acid bacteria
MESH: Chromatography, Liquid
Lactobacillus plantarum
Biologie végétale
Chromatography, Liquid
[SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Subjects
Details
- Language :
- English
- ISSN :
- 14712180
- Database :
- OpenAIRE
- Journal :
- BMC Microbiology (11), 63. (2011), BMC Microbiology, BMC Microbiology, BioMed Central, 2011, 11, pp.63. ⟨10.1186/1471-2180-11-63⟩, BMC Microbiology, 11:63. BMC, BMC Microbiology, Vol 11, Iss 1, p 63 (2011)
- Accession number :
- edsair.doi.dedup.....26efd2700b24f78c19a3353afd77edfb