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Anti-LPS IgA and IgG Can Inhibit Serum Killing of Pseudomonas aeruginosa in Patients with Cystic Fibrosis
Anti-LPS IgA and IgG Can Inhibit Serum Killing of Pseudomonas aeruginosa in Patients with Cystic Fibrosis
- Source :
- Infect Immun
- Publication Year :
- 2021
- Publisher :
- American Society for Microbiology, 2021.
-
Abstract
- Pseudomonas aeruginosa is one of the principal pathogens implicated in respiratory infections of patients with cystic fibrosis (CF) and non-CF bronchiectasis. Previously, we demonstrated that impaired serum-mediated killing of P. aeruginosa was associated with increased severity of respiratory infections in patients with non-CF bronchiectasis. This inhibition was mediated by high titres of O-antigen-specific IgG2 antibodies that cloak the surface of the bacteria, blocking access to the membrane. Infection related symptomatology was ameliorated in patients by using plasmapheresis to remove the offending antibodies. To determine if these inhibitory 'cloaking antibodies' were prevalent in patients with CF, we investigated 70 serum samples from patients with P. aeruginosa infection, and five without P. aeruginosa infection. Thirty-two percent of patients had serum that inhibited the ability of healthy control serum to kill P. aeruginosa. Here we demonstrate that this inhibition of killing requires O-antigen expression. Furthermore, we reveal that while IgG alone can inhibit the activity of healthy control serum, O-antigen specific IgA in patient sera can also inhibited serum-killing. We found that antibody affinity, not just titre, was also important in the inhibition of serum-mediated killing. These studies provide novel insight into cloaking antibodies in human infection and may provide further options in CF and other diseases for treatment of recalcitrant P. aeruginosa infections.
- Subjects :
- Lipopolysaccharides
Cystic Fibrosis
Lipopolysaccharide
medicine.medical_treatment
Immunology
Biology
medicine.disease_cause
Microbiology
Cystic fibrosis
chemistry.chemical_compound
medicine
Humans
Pseudomonas Infections
Respiratory system
Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Bronchiectasis
Pseudomonas aeruginosa
Complement System Proteins
medicine.disease
Antibodies, Bacterial
Immunoglobulin A
Titer
Infectious Diseases
chemistry
Immunoglobulin G
Host-Pathogen Interactions
biology.protein
Parasitology
Plasmapheresis
Antibody
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....26e9666e9a70e85f7c9428753c1aaa7f
- Full Text :
- https://doi.org/10.1128/iai.00412-21