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Embryo-dependent induction of embryo receptivity in the mouse endometrium
- Source :
- Journal of reproduction and fertility. 115(2)
- Publication Year :
- 1999
-
Abstract
- The effect of intraoviductal embryos on endometrial receptivity was studied by intraendometrial and intrauterine embryo transfer. Five-week-old female ICR mice were mated after superovulation; a vaginal plug confirmed day 1 of pregnancy. On day 4 (90 h after hCG injection), blastocysts were collected and transferred to pseudopregnant female mice and to recipient mice in which the uterotubal junction had been ligated bilaterally on day 1 of pregnancy. Three embryos per uterine horn, a total of six embryos per recipient mouse at days 1-6, were transferred to the endometrium or uterine cavity and implantation and pregnancy rates were calculated. The implantation rate for intraendometrial embryo transfer to recipients of days 3, 5 and 6 was significantly higher for uterotubal junction-ligated mice (72.2, 20.8 and 9.7%, respectively) than for pseudopregnant mice (55.0, 8.3 and 0.0%, respectively). The implantation rate for intrauterine embryo transfer to recipients at days 2, 5 and 6 was significantly higher for uterotubal junction-ligated mice (11.1, 25.0 and 8.3%, respectively) than for pseudopregnant mice (0.0, 3.3 and 0.0%, respectively). Uterotubal junction-ligated mice achieved implantation and bore neonates by intrauterine embryo transfer on days 2 and 6, whereas no implantation was achieved in pseudopregnant mice. The difference in implantation rate could not be explained by a difference in progesterone concentration between the groups. The distribution of proliferating cells in the endometrium was also studied immunohistochemically by use of anti-proliferating cell nuclear antigen (PCNA) antibody in the recipient mice. PCNA-positive cells were more abundant in uterotubal junction-ligated mice and demonstrated a marked extension from the epithelium to the stroma over time, in contrast to those in pseudopregnant mice. These findings indicate that an intraoviductal embryo exerts a biological effect by sending a signal to the endometrial epithelium and stroma, thus facilitating endometrial receptivity to the embryo and improving the rate of implantation.
- Subjects :
- Embryology
medicine.medical_specialty
Ratón
Biology
Endometrium
Chorionic Gonadotropin
Statistics, Nonparametric
Mice
Endocrinology
Pregnancy
Internal medicine
Proliferating Cell Nuclear Antigen
medicine
Animals
Embryo Implantation
Uterotubal junction
Pseudopregnancy
Progesterone
Mice, Inbred ICR
Obstetrics and Gynecology
Embryo
Uterine horns
Cell Biology
medicine.disease
Embryo Transfer
Embryo, Mammalian
Immunohistochemistry
Embryo transfer
medicine.anatomical_structure
Reproductive Medicine
Gestation
Female
Subjects
Details
- ISSN :
- 00224251
- Volume :
- 115
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of reproduction and fertility
- Accession number :
- edsair.doi.dedup.....26e84990f5e935bb3e2518188ef87187