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Chronic UVR causes increased immunostaining of CD44 and accumulation of hyaluronan in mouse epidermis
- Source :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 59(10)
- Publication Year :
- 2011
-
Abstract
- Chronic intense UV radiation is the main cause of epidermal tumors. Because hyaluronan (HA), a large extracellular polysaccharide, is known to promote malignant growth, hyaluronan expression was studied in a model in which long-term UV radiation (UVR) induces epidermal tumors. Mouse back skin was exposed three times a week for 10.5 months to UVR corresponding to one minimal erythema dose, processed for histology, and stained for hyaluronan and the hyaluronan receptor CD44. This exposure protocol caused epidermal hyperplasia in most of the animals; tumors, mainly squamous cell carcinomas (SCCs), were found in ~20% of the animals. Specimens exposed to UVR showed increased hyaluronan and CD44 staining throughout the epidermal tissue. In hyperplastic areas, hyaluronan and CD44 stainings correlated positively with the degree of hyperplasia. Well-differentiated SCCs showed increased hyaluronan and CD44 staining intensities, whereas poorly differentiated tumors and dysplastic epidermis showed areas where HA and CD44 were locally reduced. The findings indicate that HA and CD44 increase in epidermal keratinocytes in the premalignant hyperplasia induced by UV irradiation and stay elevated in dysplasia and SCC, suggesting that the accumulation of hyaluronan and CD44 is an early marker for malignant transformation and may be a prerequisite for tumor formation.
- Subjects :
- Keratinocytes
Pathology
medicine.medical_specialty
Histology
Neoplasms, Radiation-Induced
Skin Neoplasms
Ultraviolet Rays
Article
Malignant transformation
chemistry.chemical_compound
Mice
Hyaluronic acid
medicine
Animals
Hyaluronic Acid
Hyperplasia
Epidermis (botany)
biology
integumentary system
CD44
medicine.disease
Hyaluronan-mediated motility receptor
Hyaluronan Receptors
chemistry
Erythema
biology.protein
Female
Anatomy
Epidermis
Precancerous Conditions
Immunostaining
Subjects
Details
- ISSN :
- 15515044
- Volume :
- 59
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- Accession number :
- edsair.doi.dedup.....26de88ee279e1ab12294141385700ef3