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Hyaluronan production enhances shedding of plasma membrane-derived microvesicles
- Source :
- Experimental Cell Research. 319:2006-2018
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Many cell types secrete plasma membrane-bound microvesicles, suggested to play an important role in tissue morphogenesis, wound healing, and cancer spreading. However, the mechanisms of their formation have remained largely unknown. It was found that the tips of long microvilli induced in cells by overexpression of hyaluronan synthase 3 (HAS3) were detach into the culture medium as microvesicles. Moreover, several cell types with naturally active hyaluronan synthesis released high numbers of plasma membrane-derived vesicles, and inhibition of hyaluronan synthesis reduced their formation. The vesicles contained HAS, and were covered with a thick hyaluronan coat, a part of which was retained even after purification with high-speed centrifugation. HAS3 overexpressing MDCK cells cultured in a 3-D matrix as epithelial cysts released large amounts of HAS- and hyaluronan-positive vesicles from their basal surfaces into the extracellular matrix. As far as we know, hyaluronan synthesis is one of the first molecular mechanisms shown to stimulate the production of microvesicles. The microvesicles have a potential to deliver the hyaluronan synthase machinery and membrane and cytoplasmic materials to other cells, influencing tissue regeneration, inflammation and tumor progression.
- Subjects :
- Male
Green Fluorescent Proteins
Cell Culture Techniques
Coated Vesicles
Biology
Matrix (biology)
Transfection
Extracellular matrix
Dogs
Animals
Humans
Secretion
Glucuronosyltransferase
Hyaluronic Acid
Rats, Wistar
Cells, Cultured
Vesicle
Microvesicle
Cell Membrane
Cell Biology
Microvesicles
Rats
Cell biology
Hyaluronan synthase
Biochemistry
biology.protein
Wound healing
Hyaluronan Synthases
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 319
- Database :
- OpenAIRE
- Journal :
- Experimental Cell Research
- Accession number :
- edsair.doi.dedup.....26d8e7dce934d376f2ad9fba466b6735