Additional file 4 of An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis

Additional file 4: Table S1. Specificity of the emm75 qPCR. S. pyogenes from different emm-clusters and other respiratory pathogens were used to demonstrate the specificity of the emm75 qPCR. Each value is the mean of duplicate reactions. Samples with no provided Ct value failed to cross the fluorescence threshold by the 40th (last) cycle of the assay and all samples with Ct values greater than 35 were considered negative. No template control was included and this did not cross the fluorescence threshold by the 40th cycle.