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Non-eosinophilic Airway Hyper-reactivity in Mice, Induced by IFN-γProducing CD4+and CD8+Lung T cells, is Responsive to Steroid Treatment
- Source :
- Scandinavian Journal of Immunology. 80:327-338
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- Non-eosinophilic asthma is characterized by infiltration of neutrophils into the lung and variable responsiveness to glucocorticoids. The pathophysiological mechanisms have not been characterized in detail. Here, we present an experimental asthma model in mice associated with non-eosinophilic airway inflammation and airway hyper-responsiveness (AHR). For this, BALB/c mice were sensitized by biolistic DNA immunization with a plasmid encoding the model antigen β-galactosidase (pFascin-βGal mice). For comparison, eosinophilic airway inflammation was induced by subcutaneous injection of βGal protein (βGal mice). Intranasal challenge of mice in both groups induced AHR to a comparable extent as well as recruitment of inflammatory cells into the airways. In contrast to βGal mice, which exhibited extensive eosinophilic infiltration in the lung, goblet cell hyperplasia and polarization of CD4(+) T cells into Th2 and Th17 cells, pFascin-βGal mice showed considerable neutrophilia, but no goblet cell hyperplasia and a predominance of Th1 and Tc1 cells in the airways. Depletion studies in pFascin-βGal mice revealed that CD4(+) and CD8(+) cells cooperated to induce maximum inflammation, but that neutrophilic infiltration was not a prerequisite for AHR induction. Treatment of pFascin-βGal mice with dexamethasone before intranasal challenge did not affect neutrophilic infiltration, but significantly reduced AHR, infiltration of monocytes and lymphocytes as well as content of IFN-γ in the bronchoalveolar fluid. Our results suggest that non-eosinophilic asthma associated predominantly with Th1/Tc1 cells is susceptible to glucocorticoid treatment. pFascin-βGal mice might represent a mouse model to study pathophysiological mechanisms proceeding in the subgroup of asthmatics with non-eosinophilic asthma that respond to inhaled steroids.
- Subjects :
- Neutrophils
Immunology
Inflammation
Biology
Lymphocyte Activation
Dexamethasone
Lymphocyte Depletion
Interferon-gamma
Mice
Th2 Cells
Antigen
medicine
Animals
Lung
Mice, Inbred BALB C
DNA
General Medicine
Biolistics
Th1 Cells
respiratory system
beta-Galactosidase
medicine.disease
Asthma
Neutrophilia
respiratory tract diseases
Eosinophils
Disease Models, Animal
medicine.anatomical_structure
Neutrophil Infiltration
Th17 Cells
Female
Goblet Cells
medicine.symptom
Bronchoalveolar Lavage Fluid
Infiltration (medical)
CD8
Glucocorticoid
T-Lymphocytes, Cytotoxic
medicine.drug
Subjects
Details
- ISSN :
- 03009475
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- Scandinavian Journal of Immunology
- Accession number :
- edsair.doi.dedup.....26b6b558185b3de6961c7ce534b43934